Original title: P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE
Authors: Haroniková, Lucia ; Špaček, Jan ; Fojta, Miroslav
Document type: Papers
Conference/Event: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Year: 2014
Language: eng
Abstract: In this work, we present a new qualitative approach of detection of PCR products using electrochemistry on pencil electrodes. PCR products with an incorporated biotin-labeled dNTP (dCTP in this study) are detected via conjugated streptavidinealkaline phospatase. 1-Naphthyl phosphate (1-NP), which is dephosphorylated by alkaline phosphatase to release 1-naphthol, was used as a substrate in electrochemical detection of the PCR product. Voltammetric measurement on disposable pencil electrode is a very cheap and easy to use method. The system was optimized for plasmid DNA PCR product with potential to detect human genome DNA products and possible application in gene deletion monitoring.
Keywords: DNA
Host item entry: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297918

Permalink: http://www.nusl.cz/ntk/nusl-396435


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Research > Institutes ASCR > Institute of Biophysics
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