TY - GEN TI - P36 VOLTAMETRIC DETECTION OF DNA DELETION ON PENCIL ELECTRODE T3 - CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./ AU - Haroniková, Lucia AB - In this work, we present a new qualitative approach of detection of PCR products using electrochemistry on pencil electrodes. PCR products with an incorporated biotin-labeled dNTP (dCTP in this study) are detected via conjugated streptavidinealkaline phospatase. 1-Naphthyl phosphate (1-NP), which is dephosphorylated by alkaline phosphatase to release 1-naphthol, was used as a substrate in electrochemical detection of the PCR product. Voltammetric measurement on disposable pencil electrode is a very cheap and easy to use method. The system was optimized for plasmid DNA PCR product with potential to detect human genome DNA products and possible application in gene deletion monitoring. SN - 978-80-904959-2-0 UR - http://hdl.handle.net/11104/0297918 UR - http://www.nusl.cz/ntk/nusl-396435 A2 - Fojta, Miroslav A2 - Špaček, Jan LA - eng KW - DNA PY - 2014 PB - Biofyzikální ústav, Královopolská 135, 612 65 Brno, http://www.ibp.cz/ ER -