Original title: Electrochemical Analysis of Chemically Modified DNA at Gold Electrodes
Authors: Vidláková, Pavlína ; Havran, Luděk ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year: 2016
Language: eng
Abstract: DNA labeling is used to increase sensitivity of its electrochemical detection. Covalently bound markers based on a complex of osmium tetroxide with 22-bipyridine(Os,bipy) is one of those frequently applied. Os bipy reacts with pyrimidines (mainly thymines) in single stranded regions of DNA, therefore it represents a useful marker for the analysis of DNA structure or labeling of hybridization probes . Another possibility of DNA labeling is the incorporation of chemically modified dNTPs into DNA molecules with using primer extension. DNA labeling in connection with voltammetry at gold electrodes has application potential in development of sensors for studying of DNA structure and interaction.
Keywords: enzymatic incorporation; immobilization; nucleic-acids
Project no.: GA15-08434S (CEP), GBP206/12/G151 (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297888

Permalink: http://www.nusl.cz/ntk/nusl-396411


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers
 Record created 2019-07-25, last modified 2020-03-27


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