TY - GEN TI - Electrochemical Analysis of Chemically Modified DNA at Gold Electrodes T3 - Moderní elektrochemické metody /36./ AU - Vidláková, Pavlína AB - DNA labeling is used to increase sensitivity of its electrochemical detection. Covalently bound markers based on a complex of osmium tetroxide with 22-bipyridine(Os,bipy) is one of those frequently applied. Os bipy reacts with pyrimidines (mainly thymines) in single stranded regions of DNA, therefore it represents a useful marker for the analysis of DNA structure or labeling of hybridization probes . Another possibility of DNA labeling is the incorporation of chemically modified dNTPs into DNA molecules with using primer extension. DNA labeling in connection with voltammetry at gold electrodes has application potential in development of sensors for studying of DNA structure and interaction. SN - 978-80-905221-4-5 UR - http://www.nusl.cz/ntk/nusl-396411 UR - http://hdl.handle.net/11104/0297888 A2 - Fojta, Miroslav A2 - Havran, Luděk LA - eng KW - enzymatic incorporation KW - nucleic-acids KW - immobilization PY - 2016 PB - Biofyzikální ústav, Královopolská 135, 612 65 Brno, http://www.ibp.cz/ ER -