Název:
Construction and Application of Flow Amperometric Biosensor Based on Enzymatic Reactor for Determination of Choline
Autoři:
Tvorynska, Sofiia ; Barek, J. ; Josypčuk, Bohdan Typ dokumentu: Příspěvky z konference Konference/Akce: Moderní elektrochemické metody /39./, Jetřichovice (CZ), 20190520
Rok:
2019
Jazyk:
eng
Abstrakt: In this contribution, a novel fast, simple and stable biosensor with the enzymatic reactor based on choline oxidase (ChOx) was developed and applied for the determination of choline using flow injection analysis with amperometric detection. The preparation of the reactor is based on the covalent immobilization of ChOx with glutaraldehyde to mesoporous silica powder (SBA-15) previously covered by NH2-groups. The experimental parameters affecting the sensitivity and stability of the biosensor were optimized. The proposed biosensor with the newly developed ChOx-based reactor possesses good repeatability, reproducibility, long-term stability, and reusability. It was successfully applied for the determination of choline in commercial pharmaceuticals.
Klíčová slova:
choline; choline oxidase; enzymatic reactor; Flow analysis Číslo projektu: GA17-05387S (CEP) Poskytovatel projektu: GA ČR Zdrojový dokument: XXXIX. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-7-6
Instituce: Ústav fyzikální chemie J. Heyrovského AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný na vyžádání prostřednictvím repozitáře Akademie věd. Původní záznam: http://hdl.handle.net/11104/0296751