Original title: Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Authors: Dvořák, Miloš ; Miková, B. ; Šlampová, Andrea ; Kubáň, Pavel
Document type: Papers
Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year: 2018
Language: eng
Abstract: Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L.
Keywords: dried blood spots; hollow fibre-liquid phase microextraction; microextraction of basic drugs
Project no.: GA18-13135S (CEP), GA16-09135S (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Original record: http://hdl.handle.net/11104/0290091

Permalink: http://www.nusl.cz/ntk/nusl-390107


The record appears in these collections:
Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers