Název:
Thickness determination of a cathodoluminescence active nanoparticles by means of Quantitative STEM imaging
Autoři:
Skoupý, Radim ; Krzyžánek, Vladislav Typ dokumentu: Příspěvky z konference Konference/Akce: Recent Trends in Charged Particle Optics and Surface Physics Instrumentation, Skalský dvůr (CZ), 20180604
Rok:
2018
Jazyk:
eng
Abstrakt: Labeling of specimens by nanoscale probes is common approach of complex biological\nsystems exploration. Namely gold nanoparticles immuno-staining is well established method\nin electron microscopy. However, if more than two label sizes are used, the differentiation of\nindividual nanoparticles becomes difficult.\nThis can be overcome by cathodoluminescence (CL) active particles – nanophosphors where\nlabels recognition is done by wavelength of emitted light. This gives a great opportunity to\nuse advanced multi probe labeling within one sample.\nThere is a huge variety of nanophosphors: green fluorescent protein, quantum dots, ZnO\nnanoparticles, organic molecules, rare earth-doped nanophosphors etc. Therefore, in order\nto choose best type of nanophosphors for a given task, it is important to measure particles\nsize/thickness, as the CL intensity is proportional to the probe volume.
Klíčová slova:
Monte Carlo; quantitative imaging Číslo projektu: GA17-15451S (CEP), LO1212 (CEP), ED0017/01/01 Poskytovatel projektu: GA ČR, GA MŠk, GA MŠk Zdrojový dokument: Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Proceedings of the 16th International Seminar, ISBN 978-80-87441-23-7
Instituce: Ústav přístrojové techniky AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0287632