Original title: LP-12 Approaches to visualization of 3D structures captured by a confocal microscope
Translated title: Přístupy k visualizaci obrazů 3D struktur získaných konfokálním mikroskopem
Authors: Čapek, Martin ; Janáček, Jiří ; Kubínová, Lucie ; Hána, K. ; Smrčka, P.
Document type: Papers
Conference/Event: Multinational Congress on Microscopy /8./, Prague (CZ), 2007-06-17 / 2007-06-21
Year: 2007
Language: eng
Abstract: [eng] [cze]
Laser scanning confocal microscopes are capable to focus a laser beam into a layer of an investigated biological specimen, and by the gradual scanning of this layer they acquire an optical section. By consecutive scanning of all preset layers of the specimen we obtain a stack of optical sections, i.e. a 3D digital representation of the specimen. In the presented study we focus on volume reconstruction of large biological tissues, i.e. tissues greater than field of view and/or thicker than maximal depth of scanning of a confocal microscope. As a result of volume reconstruction we obtain a high resolution 3D image of the biological specimen. 3D visualization is offered either by our Rapid3D software package suited for three-dimensional reconstruction and visualization of biomedical images, or Ellipse modular software package devoted to biological image processing (created by ViDiTo company, Slovakia) Článek popisuje přístupy k objemové rekonstrukci a vizualizaci 3D biologických struktur nasnímaných konfokálním mikroskopem
Keywords: confocal microscopy; volume visualization
Project no.: CEZ:AV0Z50110509 (CEP), IAA100110502 (CEP), IAA500200510 (CEP), GA304/05/0153 (CEP), LC06063 (CEP)
Funding provider: GA AV ČR, GA AV ČR, GA ČR, GA MŠk
Host item entry: Proceedings 8th multinational congress on microscopy, ISBN 978-80-239-9397-4

Institution: Institute of Physiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0149164

Permalink: http://www.nusl.cz/ntk/nusl-37491


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Research > Institutes ASCR > Institute of Physiology
Conference materials > Papers