Original title: Rodina proteinů obsahujících sterol-sensing doménu
Translated title: Rodina proteinů obsahujících sterol-sensing doménu
Authors: Ditrychová, Karolína ; Zíková, Martina (advisor) ; Fafílek, Bohumil (referee)
Document type: Bachelor's theses
Year: 2014
Language: eng
Abstract: [eng] [cze]
1. Abstract Sterol sensing domain (SSD) is a putative transmembrane region consisting of 5 helices and in few cases it was shown that it interacts with cholesterol. Proteins containing this domain play a role in many cellular pathways connected to cholesterol. It was first described in a study of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase). It is an enzyme playing role in cholesterol biosynthesis and its transcription is regulated by sterol regulatory element binding protein cleavage activating protein (SCAP). HMG-CoA reductase and SCAP were the first proteins where SSD was recognized. After that, other proteins were described; one more protein functioning in cholesterol biosynthesis was described as containing SSD - 7-dehydrocholesterol reductase. Also two proteins playing role in cholesterol endocytosis - Niemann-Pick type C 1 liked 1 - and in its efflux from lysosomes - Niemann-Pick type C 1 - were recognized. Furthermore, proteins playing role in Hedgehog signalling Patched, Patched-related and Dispatched were found as SSD containing structures. In the end, tumour-suppressor gene TRC8 was also recognized as member of SSD family. In this text, structure and function of these proteins is summarized. Key words: sterol sensing domain, Hedgehog signalling pathway, sterol binding protein,... Sterol sensing doména (SSD) je transmembránový úsek proteinu skládající se z 5 helixů. Proteiny, které tuto doménu obsahují, jsou součástí mnoha buněčných drah souvisejících s metabolismem cholesterolu. V některých případech bylo ukázáno, že SSD interaguje s molekulou cholesterolu. Poprvé byla popsána během studia 3-hydroxy-3-metylglutaryl-CoA reduktázy (HMG-CoA reduktáza), enzymu účastnícího se biosyntézy cholesterolu. Jeho transkripce je regulována proteinem Sterol regulatory element binding protein Cleavage Activating Protein (SCAP). 3HMG-CoA reduktáza a SCAP byly prvními proteiny, v jejichž struktuře byla SSD rozeznána. Později byl popsán jiný protein s touto doménou, který je také součástí biosyntézy cholesterolu - 7-dehydrocholesterol reduktáza. Další proteiny s identifikovanou SSD, hrají roli v endocytóze cholesterolu a jeho transportu z lysozomů - Niemann-Pick typ C 1 liked 1 a Niemann-Pick typ C 1. Mimoto, jako struktury obsahující SSD byly také popsány proteiny působící v Hedgehogové signalizaci - Patched, Patched-related a Dispatched. Konečně, protein působící jako tumor-supresor TRC8 byl do této rodiny proteinů zařazen také. V této práci je popsána struktura a funkce jednotlivých členů proteinové rodiny obsahující sterol sensing doménu. Klíčová slova: sterol sensing doména, signální...
Keywords: cholesterol synthesis; cholesterol trafficking; Hedgehog signalling pathway; sterol binding protein; sterol sensing domain; proteiny vázající sterol; signální dráha Hedgehog; sterol sensing doména; syntéza cholesterolu; transport cholesterolu

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/68766

Permalink: http://www.nusl.cz/ntk/nusl-337902


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Bachelor's theses