Original title:
Biotechnologická produkce vybraných metabolitů na syrovátkovém substrátu
Translated title:
Biotechnological production of selected metabolites on whey substrate
Authors:
Gadová, Martina ; Kočí, Radka (referee) ; Márová, Ivana (advisor) Document type: Master’s theses
Year:
2017
Language:
slo Publisher:
Vysoké učení technické v Brně. Fakulta chemická Abstract:
[slo][eng]
Predložená diplomová práca sa zaoberá posúdením možnosti biotechnologického využitia odpadu, srvátky. Skúma obsahové zloženie lyofilizovanej srvátky, optimalizáciou kyslej hydrolýzy srvátky a následnej prípravy kultivačných médií s rôznym obsahom a úpravou srvátky. Cieľom práce je aj screening mikrobiálnych producentov biotechnologicky zaujímavých metabolitov z hľadiska ich rastu a produkcie vybraného metabolitu na srvátkových médiách. Substráty a vyprodukované metabolity boli analyzované pomocou UHPLC-PDA-RI a GC-FID a množstvo mikrobiálnych lipidov bolo stanovené grafimetricky. Boli študované kvasinky rodu Saccharomyces, Metschnikowia a baktérie rodu Lactobacillus za účelom produkcie etanolu, mikrobiálnych lipidov a kyseliny mliečnej. Najvyššia produkcia sa u kvasiniek dosiahla na srvátkovom médiu obsahujúcom hydrolyzovanú laktózu a u baktérii srvátkové médium bez hydrolýzy.
The submitted master thesis deals with assessment of the possibility of using of cheese whey for biotechnological production. Study content composition of lyophilized whey, optimization of acid hydrolysis and preparation of cultivating mediums with different content and treatment of cheese whey. Between the aims of this thesis belong also screening of microbial producers, interesting for biotechnology and looks at their growth and production of selected metabolites of cultivation mediums containing cheese whey. All substrates and produced metabolites where examined with UHPLC-PDA-RI and GC-FID: Amount of produced microbial lipids was determined by gravimetric analysis. Examined microorganisms belonged to yeast genus Saccharomyces, Metschnikowia and bacterial genus Lactobacillus and its focused on production of ethanol, microbial lipids and lactic acid. The highest yields with using yeasts were obtained using production medium containing hydrolysed lactose in cheese whey. The highest production, in case of bacteria, was obtained using non-hydrolysed, untreated cheese whey production medium.
Keywords:
acid hydrolysis; bacteria; Cheese whey; ethanol; lactic acid; lipids; yeast
Institution: Brno University of Technology
(web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library. Original record: http://hdl.handle.net/11012/65969