Original title:
Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy
Authors:
KOČOVÁ, Pavlína Document type: Master’s theses
Year:
2017
Language:
cze Abstract:
This study is focused on changes in protein expression in a glioblastoma cell line during infection with tick-borne encephalitis virus. Newly synthesized proteins were distinguished from previously synthesized proteins using bioorthogonal chemistry (BONCAT method) to observe changes in protein synthesis. Labelled proteins were visualized using two-dimensional PAGE and western blotting followed by Click reaction on membrane. Differences in protein pattern between control and infected cells were observed.
Keywords:
2D PAGE; bioorthogonal chemistry; BONCAT; tick-borne encephalitis; 2D PAGE; bioortogonální chemie; BONCAT; klíšťová encefalitida Citation: KOČOVÁ, Pavlína. Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy. České Budějovice, 2017. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/40494