Název: Charakteristika programované buněčné smrti při vzniku lyzigenního aerenchymu
Překlad názvu: Characterization of programmed cell death during lyzigenous aerenchyma formation
Autoři: Lenochová, Zuzana ; Votrubová, Olga (vedoucí práce) ; Čiamporová, Milada (oponent) ; Kummerová, Marie (oponent)
Typ dokumentu: Disertační práce
Rok: 2012
Jazyk: cze
Abstrakt: Abstract____________________________________________________________________ 2 ABSTRACT: Zea mays is generally considered to be a plant with inducible lysigenous aerenchyma formation. The degradation of some cortical cells is triggered by environmental conditions, usually in the form of stress (submergence etc.). These cells die in a process that shows signs characteristic for programmed cell death, such as nuclear DNA fragmentation or apoptotic ultrastructural alterations. Aerenchyma formed in primary roots of thirteen examined maize accessions, irrespective of cultivation conditions. The aerenchyma fraction correlated with the root length, not with its age. The dependence of aerenchyma formation on the presence of this phytohormone was proved by using an inhibitor of ethylene synthesis (AOA). It was found out that the aerenchyma formation depended on light conditions and that the lysigenous intercellulars intercellular spaces colocalized with areas with cells with characteristically fragmented nuclear DNA (TUNEL-positive nuclei). In experiments using the TUNEL reaction it was necessary to determine new dilution of the enzymatic mixture for the examined plant material. Only the observation of surface planes of free-hand root sections was considered relevant in both TUNEL-TMR and TUNEL-AP assays. TUNEL-AP...
Klíčová slova: aerenchym; kořeny; kukuřice; mokřadní rostlny; PCD; aerenchyma; maize; PCD; roots; wetland plants

Instituce: Fakulty UK (VŠKP) (web)
Informace o dostupnosti dokumentu: Dostupné v digitálním repozitáři UK.
Původní záznam: http://hdl.handle.net/20.500.11956/45189

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-309479


Záznam je zařazen do těchto sbírek:
Školství > Veřejné vysoké školy > Univerzita Karlova > Fakulty UK (VŠKP)
Vysokoškolské kvalifikační práce > Disertační práce