Název: Charakterizace membránového proteinu DREPP
Překlad názvu: Characterization of membrane protein DREPP
Autoři: Vosolsobě, Stanislav ; Schwarzerová, Kateřina (vedoucí práce) ; Cvrčková, Fatima (oponent)
Typ dokumentu: Diplomové práce
Rok: 2010
Jazyk: cze
Abstrakt: Proteins of DREPP family (20-25 kDa, syn. PCaP1 in Arabidopsis thaliana) first appeared in ferns and we have shown that several independent duplications of DREPP protein occurred during evolution of large families (Poaceae, Brassicaceae, Solanaceae and Asteraceae) and in group Coniferophyta. Secondary losses of one paralogue occurred in subfamilies Pooideae and Solanoideae.We have also detected two large-scale modification of DREEP protein in Asparagales and Brassicaceae (this divergent paralogue was previously described as MAP18 protein). We have examined colinearity of chromosome fragments in vicinity both PCaP1 and MAP18 paralogues in Arabidopsis thaliana and we hypothesize that MAP18 gene arose during genome duplication on the origin of Brassicaceae family. DREPP protein was previously identified in detergent-resistant membrane microdomain fraction and a myristyl anchor was shown to be necessary for their membrane localization. Membrane association was shown to be modified by the interaction of unique N-terminal domain with PtdInsPs, which was inhibited by binding of Ca-calmodulin (Nagasaki et al., 2008). The mutation of Gly2 by Ala in the myristilation site, or C-terminal GFP-fusion (GFP-DREPP), affect membrane association in Arabidopsis thaliana (Nagasaki et al., 2008). Several DREPP paralogues in...
Klíčová slova: cytoskelet; huseníček; mikrotubuly; plasmatická membrána; tabák; Arabidopsis thaliana; biolistic; developmentally regulated plasma membrane polypeptide; DREPP; GFP fusion; MAP18; microtubul; microtubule-asociated protein; Nicotiana tabacum; PCaP1; PCaP2; plasma membrane; plasma membrane-asociated cation-binding protein

Instituce: Fakulty UK (VŠKP) (web)
Informace o dostupnosti dokumentu: Dostupné v digitálním repozitáři UK.
Původní záznam: http://hdl.handle.net/20.500.11956/31373

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-295860


Záznam je zařazen do těchto sbírek:
Školství > Veřejné vysoké školy > Univerzita Karlova > Fakulty UK (VŠKP)
Vysokoškolské kvalifikační práce > Diplomové práce