Original title: Molecular cloning, E.coli expression and purification of SCFV antibody fragments of diagnostic/therapeutic interest
Authors: Král, Vlastimil ; Fábry, Milan ; Hořejší, Magdalena ; Závada, Jan ; Sedláček, Juraj
Document type: Papers
Conference/Event: Meeting of the Czech and Slovak Structural Biologists /2./, Nové Hrady (CZ), 2003-03-13 / 2003-03-15
Year: 2003
Language: eng
Abstract: We describe molecular cloning, expression, purification and properties of two single chain antibody variable fragments (scFv) of potential diagnostic use, namely scFv M75 and scFv Tu-20. The former scFv is derived from a monoclonal antibody M75 specific for a cell surface protein MN/CA IX, strongly associated with many types of human carcinomas. The latter scFv is derived from a monoclonal antibody TU-20 specific for neuronal beta-III-tubulin.
Keywords: cloning; purification; scFv
Project no.: CEZ:AV0Z5052915 (CEP), GV203/98/K023 (CEP)
Funding provider: GA ČR
Host item entry: Material Structure in Chemistry, Biology, Physics and Technology, ISSN 1211-5894

Institution: Institute of Molecular Genetics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0087284

Permalink: http://www.nusl.cz/ntk/nusl-28135


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Research > Institutes ASCR > Institute of Molecular Genetics
Conference materials > Papers