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Original title:
Antigenic and enzymatic properties of sand fly saliva
Translated title: Antigenic and enzymatic properties of sand fly saliva
Authors: Hostomská, Jitka ; Volf, Petr (advisor) ; Salát, Jiří (referee) ; Bilej, Martin (referee)
Document type: Doctoral theses
Year: 2008
Language: eng
Abstract: i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/18282
Permalink: http://www.nusl.cz/ntk/nusl-272334
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses
Translated title: Antigenic and enzymatic properties of sand fly saliva
Authors: Hostomská, Jitka ; Volf, Petr (advisor) ; Salát, Jiří (referee) ; Bilej, Martin (referee)
Document type: Doctoral theses
Year: 2008
Language: eng
Abstract: i SUMMARY OF RESULTS The thesissumsup the resultsof threeprojectsI was involvedin duringmy PhD study.Specifically, I adressedthe putativeeffect of sand fly salivary hyaluronidaseon transmissionandestablishmentof kishmania infection.The secondprojectwasdealingwith thekineticsof anti-salivaantibodyresponsein dogsexposedto Lutzomyialongipalpissutd flies andwith the characterizationof salivaryantigensrecognizedby thesedogs.Finally, I constructedand annotateda cDNA library from Phlebotomusarabicas and characterized P. arabicus sďivary antigensreactingwith arrtibodiesof mice exposedto this sand fly species.Theresultsofthe projectsaÍebrieflyoutlinedhere. We detectedhyaluronidaseactivity in saliva of various bloodsuckingDipter4 includingsandflies. In coinoculationexperimentswith BALB/o mice we proveda positive conelationbetweenthe size of the cutaneouslesion causedby Leishmaniamajor andthe presenceofhyaluronidasein theinfectiveinoculum.In hyďuronidase-coinoculatedmice,the lesionsweresigniÍicantlylargerfromweek3 postinfection(infectiondosel05 parasites)or week 4 (infection dose 10"). On the other hand, hyaluronidasedid not affect early viscerďizationof L. majorat24 hrspostinfection.Thus,we demonstratedthathya|uronidase promotes Leishmania establishment in murine skin and we hypothesize that immunomodulatoryeffectsof...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/18282
Permalink: http://www.nusl.cz/ntk/nusl-272334
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses
Record created 2017-04-25, last modified 2022-03-03