Original title: Characterization of FRET sensor
Authors: Datinská, Vladimíra ; Klepárník, Karel ; Belšánová, B. ; Minárik, M. ; Foret, František
Document type: Papers
Conference/Event: CECE 2016. International Interdisciplinary Meeting on Bioanalysis /13./, Brno (CZ), 20161017
Year: 2016
Language: eng
Abstract: In this study, we present characterization of sensor based on Fӧrster resonance energy\ntransfer (FRET). The sensor is composed of ssDNA chain attached to a laboratory\nsynthesized quantum dot (QD). A complementary chain of a sample is labeled by a\nluminescent dye. When the dsDNA hybrid is formed, the energy from the QD (donor)\nis transferred to the dye (acceptor) and FRET is observed as a decrease of QD\nluminescence emission intensity and an increase of dye luminescence emission\nintensity.
Keywords: FRET sensor; Fӧrster resonance energy transfer; quantum dot
Project no.: TA02010672 (CEP), GA14-28254S (CEP)
Funding provider: GA TA ČR, GA ČR
Host item entry: CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-4-4

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0265749

Permalink: http://www.nusl.cz/ntk/nusl-261688


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers