Original title: Sperm protein profiles of different mammalian species
Authors: Pohlová, Alžběta ; Zigo, Michal ; Jonáková, Věra ; Postlerová, Pavla
Document type: Papers
Conference/Event: XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION, Třešť (CZ), 2015-05-14 / 2015-05-16
Year: 2015
Language: eng
Abstract: Proteins are a substantial equipment of the spermatic cell; therefore, the characterization of sperm proteins is crucial for explanation of molecular mechanisms in the reproduction process. We isolated sperm proteins from different mammalian species - pig, bull, human, mouse, dog and cat. Extracted proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal or ejaculated sperm were compared. Additionally, we tested cross-reactivity of antibodies prepared to sperm boar proteins on spermatozoa of other mammalian species using immunofluorescent technique. Our future plan is to compare the protein profiles of sperm during their functional development (epididymal, ejaculated, capacitated) in various mammalian species and identify species-specific sperm proteins with zona pellucida binding activity.
Keywords: monoclonal antibody; SDS-electrophoresis; sperm proteins; zona pellucida binding activity
Project no.: GA P503/12/1834, GA 14-05547S, ED1.1.00/02.0109
Funding provider: GA ČR, GA ČR, GA MŠk
Host item entry: Book of abstracts of XXIst Symposium of biology and immunology of reproduction

Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0264415

Permalink: http://www.nusl.cz/ntk/nusl-261564


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Research > Institutes ASCR > Institute of Biotechnology
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