Název: Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
Autoři: BEČKOVÁ, Martina
Typ dokumentu: Disertační práce
Rok: 2016
Jazyk: cze
Abstrakt: The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
Klíčová slova: Photosystem II; Photosystem II biogenesis; Psb28; Synechocystis
Citace: BEČKOVÁ, Martina. Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803. České Budějovice, 2016. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Instituce: Jihočeská univerzita v Českých Budějovicích (web)
Informace o dostupnosti dokumentu: Plný text je dostupný v digitálním repozitáři JČU.
Původní záznam: http://www.jcu.cz/vskp/21870

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-261549


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Školství > Veřejné vysoké školy > Jihočeská univerzita v Českých Budějovicích
Vysokoškolské kvalifikační práce > Disertační práce