Název: Dynamics of mouse sperm capacitation and acrosome reaction
Autoři: Dvořáková-Hortová, Kateřina ; Frolíková, Michaela ; Děd, Lukáš ; Šebková, Nataša
Typ dokumentu: Příspěvky z konference
Konference/Akce: XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION, Třešť (CZ), 2015-05-14 / 2015-05-16
Rok: 2015
Jazyk: eng
Abstrakt: Capacitation followed by the acrosome reaction (AR), is a very complex event of molecular changes, including acrosome matrix rearrangement and actin polymerization, which mammalian sperm must undergo in the female reproductive tract in order to obtain the ability to penetrate and fertilize the egg. CD46 and β1-integrin belong to specific proteins, which are predicted to interact during molecular reorganization of capacitating sperm. The IZUMO1 as the primary fusion protein of the mammalian sperm is also involved in this dynamic network. We investigated the relationship between the Izumo, CD46 and β1 integrin relocation in the sperm head during the capacitation and AR in vitro. We have already successfully monitored by immunofluorescent labelling the dynamics of proteins CD46 and β1-integrin. The changes in the localization of these proteins associated with the AR and their mutual co-localization was observed. The original β1-integrin location in the freshly released epididymal sperm is in the acrosome and it relocates during the AR further through the sperm head compartments into the equatorial segment and over the whole sperm head. Its density over the equatorial segment is decreasing with the extended time of the AR. Also its presence in the perforatorium of the mouse sperm head is very prominent. The pattern for protein CD46 is extremely similar if not identical in both aspects such as compartment localization and time progress during capacitation and AR in vitro. The molecular interaction of CD46 and β1-integrin was investigated using the Proximity Ligation Assay and Super resolution microscopy STED. The data were statistically analysed. The newly obtained results from CD46 and β1-integrin relocation are in correlation with IZUMO1 dynamics and giving a substantial knowledge on the studied protein network rearrangement during capacitation and AR in mouse spermatozoa.
Klíčová slova: acrosome reaction; CD46 monoclonal antibody; IZUMO 1; Proximity ligation assay; sperm capacitation
Číslo projektu: GA 14-05547S, ED1.1.00/02.0109
Poskytovatel projektu: GA ČR
Zdrojový dokument: Book of abstracts of XXIst Symposium of biology and immunology of reproduction

Instituce: Biotechnologický ústav AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0262909

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-260983


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Biotechnologický ústav
Konferenční materiály > Příspěvky z konference