Original title: Transport of ribosomal RNA within the nucleolus
Authors: Staněk, David ; Koberna, Karel ; Pliss, Artem ; Čtrnáctá, Vlasta ; Malínský, Jan ; Mašata, Martin ; Večeřová, Jaromíra ; Raška, Ivan
Document type: Papers
Conference/Event: EUREM /12./, Brno (CZ), 2000-07-09 / 2000-07-14
Year: 2000
Language: eng
Abstract: In the present study, we have explored the fact that incorporation of BrU into prerRNA did not interfere with pre-rRNA processing (11) and we have investigated the transport of non - isotopically labeled RNA within the nucleolus by means of transmissiion electron microscopy and confocal laser scanning microscopy.Within 20 minutes, BrU - labeled nucleolar RNA moved from the transcription sites in nucleolar DFC to GC. Double localization of bromouridine labeled RNA and fibrillarin revealed that only a ppart of the fibrillarin rich domains were transcriptionally active.
Project no.: CEZ:AV0Z5039906 (CEP), VS96129 (CEP), GA304/00/P025 (CEP), GA304/00/1481 (CEP), 049949/Z/97/Z/JMW/JPS/CG
Funding provider: GA MŠk, GA ČR, GA ČR, Wellcome Trust grant
Host item entry: EUREM 12

Institution: Institute of Experimental Medicine AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0070411

Permalink: http://www.nusl.cz/ntk/nusl-25822


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Research > Institutes ASCR > Institute of Experimental Medicine
Conference materials > Papers