Název: 24-Epibrassinolide at Subnanomolar Concentrations Modulates Growth of Mouse Hybridoma Cells
Autoři: Franěk, František ; Kohout, Ladislav ; Eckschlager, T.
Typ dokumentu: Sborníky
Konference/Akce: Conference of Isoprenoids /20./, Liberec (CZ), 2003-10-12 / 2003-10-18
Rok: 2003
Jazyk: eng
Abstrakt: Brassinolides are known to stimulate plant growth and to posses antistresiTactivities in plants. This work was aimed at exploring possible beneficial effects of 24-epibrassinolide on cultured mammalian cells. A mouse hybridoma, i.e. a hybrid ymphocyte cell line, was cultured either in standard serum-free medium, or in diluted medium in which the cell grew inder nutritional stress. Steady-state parameters of semicon-finuous cultures conducted at 24-epibrassinolide concentra-ions from 10~16 M to 1CT9 M were evaluated. Typical effects of he agent that were observed both in standard and in diluted i [media were suppression of intracellular antibody level, increa- / ,ed value of mitochondrial membrane potential, higher frac- / ion of cells in the G(/Gl phase and lower fraction of cells in / he S phase. Alleviation of nutritional stress was observed in j (cultures conducted in diluted media. Viable cell density was j ignificantly higher at 24-epibrassinolide concentrations 10~13[ knd 10~12 M. Results of this exploratory study show that thej plant hormone 24-epibrassinolide may induce alterations of he cell division mechanism of the energetic metabolism, and of secreted protein synthesis in a mammalian cell line.
Klíčová slova: Brassinolides; cell division
Číslo projektu: IBS4055304 (CEP), GA203/01/0083 (CEP), OC 844.10, CEZ:AV0Z4055905 (CEP), CEZ:AV0Z5038910 (CEP)
Poskytovatel projektu: GA AV ČR, GA ČR, GA MŠk

Instituce: Ústav experimentální botaniky AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0069380

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-25732


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