Original title: Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques
Authors: Horká, Marie ; Růžička, F. ; Šlais, Karel
Document type: Papers
Conference/Event: FEMS Congress of European Microbiologists /1./, Ljubljana (SI), 2003-06-29 / 2003-07-03
Year: 2003
Language: eng
Abstract: Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric focusing, respectively, for a dynamic modification of the microbial samples. Using the deuterium lamp UV excitation for the on column fluorometric detection, the minimum detectable amount of the microorganisms in order of ones to tens of cells sampled on the separation capillary was achieved.
Keywords: capillary electrokinetic techniques; dynamic modification of microorganisms; fluorometric detection
Project no.: CEZ:AV0Z4031919 (CEP), IAA4031901 (CEP), IAA4031302 (CEP), GA203/02/1447 (CEP)
Funding provider: GA AV ČR, GA AV ČR, GA ČR
Host item entry: FEMS Congress of European Microbiologists. Abstract book

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0059784

Permalink: http://www.nusl.cz/ntk/nusl-24557


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers