Original title: Standards for capillary isoelectric focusing with laser induced fluorescence detection
Authors: Šlais, Karel ; Nováčková, J. ; Friedl, Z.
Document type: Papers
Conference/Event: International Symposium Separations in the BioSciences /2./, Praha (CZ), 2001-09-17 / 2001-09-20
Year: 2001
Language: eng
Abstract: Proteins, peptides or BGE ampholytes labeled with suitable dyes were previously suggested as pI markers. However, both proteins and background ampholytes have many possible reactive sites for reactive dyes and this might result in formation of heterogeneously labeled product with respect to pI. By contrast, the low molecular pI markers can be prepared with heterogeneity and greater stability than proteins and could potentially be good pI markers as found in case of UV detection. To improve the laser induced fluorescence detection, new low molecular fluorescent compounds excitable around 490 nm with suitable acidobasic and electrophoretic properties were prepared and focused in capillary IEF with photometric or fluorometric detection. The experimental setup of IEF and properties of new laser induced fluorescent pI markers are given.
Keywords: capillary isoelectric focusing; laser induced fluorescent detection; standards for cIEF
Project no.: CEZ:AV0Z4031919 (CEP), IAA4031901 (CEP)
Funding provider: GA AV ČR
Host item entry: 2nd International Symposium Separations in the BioSciences. SBS 2001, ISBN 80-7080-437-8

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0059619

Permalink: http://www.nusl.cz/ntk/nusl-24527


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers