Original title: Regulace produkce lipidů a lipidických metabolitů u kvasinek
Translated title: Regulation of production of lipids and lipid compounds in yeasts
Authors: Rapta, Marek ; Breierová, Emília (referee) ; Márová, Ivana (advisor)
Document type: Master’s theses
Year: 2015
Language: cze
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [cze] [eng]
U oleogénnych kvasiniek dochádza za vhodných podmienok k nadprodukcii a následnej akumulácii lipidov a lipidických látok. Touto vlastnosťou sa vyznačujú aj červené kvasinky, ktoré okrem lipidov produkujú aj karotenoidy – prírodné pigmenty využívané v potravinár-stve a doplnkoch stravy. Práca bola zameraná na skríning produkčných vlastností kmeňov Cystofilobasidium capitatum, Rhodotorula glutinis, Sporobolomyces roseus a Sporobolomy-ces shibatanus. Ako zdroje uhlíka boli zvolené glukóza a glycerol ako odpadný produkt pri produkcii biopalív. Najlepšie produkčné vlastnosti sa prejavili pri kmeňoch Cystofilobasidium capitatum a Rhodotorula glutinis. Pri nich došlo k zvýšeniu akumulovaných lipidov za súčasnej produkcie vyšších koncentrácii karotenoidov. Kmene boli testované metódou FTIR spektroskopie, ktorá umožňuje vysokovýkonnú, nenáročnú a presnú analýzu. Oleogenic yeasts under appropriate conditions produce and accumulate lipids and lipid-soluble metabolites in high amounts. This attribute is characteristic also for red yeasts that except lipids accumulate also carotenoids – natural pigments used in food industry and dietary supplements. The aim of this diploma thesis was designed as a comparative screening study of production properties of strains Cystofilobasidium capitatum, Rhodotorula glutinis, Sporobolomyces roseus and Sporobolomyces shibatanus. Choosen carbon sources were glucose and glycerol as waste by-product in biofuel industry. The best production properties were found in Cystofilobasidium capitatum and Rhodotorula glutinis. These two strains produced increased amounts of lipids as well as higher amounts of carotenoids. Strains were tested by FTIR spectroscopy that enables high-throughput, uncomplicated and accurate analysis.
Keywords: C/N ratio; Cystofilobasidium capitatum; FTIR spectroscopy; karotenoids; lipids; microcultivations; Rhodotorula glutinis; Sporobolomyces roseus; Sporobolomyces shibatanus; C/N pomer; Cystofilobasidium capitatum; FTIR spektroskopia; karotenoidy; lipidy; mikrokultivácie; Rhodotorula glutinis; Sporobolomyces roseus; Sporobolomyces shibatanus

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/38495

Permalink: http://www.nusl.cz/ntk/nusl-217115


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Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Master’s theses