Název:
Synthesis of core/shell quantum dots for diagnostic
Autoři:
Mihajlovic, A. Typ dokumentu: Příspěvky z konference
Jazyk:
eng
Nakladatel: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstrakt:
In this paper, synthesis of colloidal core and core/shell quantum dots (QDs) was described. First, CdTe QDs capped with glutathione, thioglycolic or mercaptopropionic acid were prepared in aqueous phase, and used for synthesis of colloidal core/shell CdTe/ZnS QDs. Core/shell QDs were used for conjugation with bovine serum albumin (BSA) or immunoglobulin G (IgG) via different crosslinkers (CDI, EDC/NHS, EDC). QDs as well as QDs-protein/antibody conjugates were characterized via UV-Vis spectroscopy and capillary electrophoresis (CE). Based on UV-Vis spectroscopy results it was found that, with increasing concentration of BSA, fluorescence intensity of QDs decreased. CE confirmed formation of QDs-BSA and QDs-IgG conjugates.
Klíčová slova:
bovine serum albumin; conjugation; glutathione; immunoglobulin G; mercaptopropionic acid; Quantum dots; thioglycolic acid Zdrojový dokument: Proceedings of the 21st Conference STUDENT EEICT 2015, ISBN 978-80-214-5148-3
Instituce: Vysoké učení technické v Brně
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Informace o dostupnosti dokumentu:
Plný text je dostupný v Digitální knihovně VUT. Původní záznam: http://hdl.handle.net/11012/43026