Original title: Voltammetric Behavior of 4-Aminophthalimide Label using Hanging Mercury Drop Electrode
Authors: Ferenčíková, Z. ; Daňhel, Aleš ; Riedl, Jan ; Hocek, Michal ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní Elektrochemické Metody /34./, Jetřichovice (CZ), 20140519
Year: 2014
Language: eng
Abstract: 4-aminophthalimide (API), covalently attached to nucleotide (dCTP or dATP) has recently beendesigned as a fluorescent label for the detection of DNA-protein interaction. However API is electrochemically reducible and therefore it is possible to use alternatively the API-modified nuclotides as DNA redox labels. The electrochemical behavior of labeled nucleotides and labeled DNA was studied using cyclic voltammetry and transfer stripping cyclic voltammetry at hanging mercury drop electrode. Observed CVs showed irreversible reduction signals of 4-aminophthalimide label without production of consequently oxidizable/reducible species.
Keywords: 4-aminophthalimide; DNA; hanging mercury drop electrode; labeled nucleotide; primer extension; voltammetry
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody, ISBN 978-80-905221-2-1

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0257029

Permalink: http://www.nusl.cz/ntk/nusl-202729


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers