Original title: Synthesis and analysis of quantum dot conjugates intended for fret sensor
Authors: Datinská, Vladimíra ; Klepárník, Karel ; Belšánová, Barbora ; Minárik, M. ; Foret, František
Document type: Papers
Conference/Event: CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./, Brno (CZ), 2015-09-21 / 2015-09-23
Year: 2015
Language: eng
Abstract: Our work is focused on synthesis of sensor based on Fӧrster resonance energy transfer (FRET), which is aimed to be used for DNA mutation detection. The sensor is based on the attachment of laboratory synthesized quantum dot (QD) to ssDNA. A complementary chain of a sample is labeled by a fluorescent dye. Thus, only if dsDNA between both complementary chains is formed, the energy from QD (donor) can be transferr ed to the dye acceptor) and FRET is observed.
Keywords: DNA mutation detection; Fӧrster resonance energy transfer; quantum dots
Project no.: TA02010672 (CEP), GA14-28254S (CEP)
Funding provider: GA TA ČR, GA ČR
Host item entry: CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-3-7

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf
Original record: http://hdl.handle.net/11104/0251151

Permalink: http://www.nusl.cz/ntk/nusl-200898


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers