Original title:
Polymer inclusion membranes open new way in practical use of in-line coupling of microextraction to CZE
Authors:
Pantůčková, Pavla ; Kubáň, Pavel ; Boček, Petr Document type: Papers Conference/Event: CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./, Brno (CZ), 2015-09-21 / 2015-09-23
Year:
2015
Language:
eng Abstract:
Polymer inclusion membranes (PIMs) have several important features, i.e., PIMs are dry and non-porous membranes, which can be prepared ahead of use and stored without noticeable deterioration in extraction performance. In-line coupling of microextractions across PIMs to commercial capillary zone electrophoresis (CZE) instrument has been demonstrated in this contribution. Purpose-made microextraction devices with PIMs were applied, which ensured complete automation of the entire analytical procedure. Practical 169 applicability of the hyphenated analytical method was demonstrated on direct extraction, injection, CZE separation and quantification of formate (the major metabolite in methanol poisoning) in clinical samples.
Keywords:
CZE; electromembrane extraction; PIMs; SLM Project no.: GA13-05762S (CEP) Funding provider: GA ČR Host item entry: CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-3-7