Original title: Enzymatic decomposition of a locked nucleoside phosphoramidate monitored by 31P NMR spectroscopy
Authors: Procházková, Eliška ; Hřebabecký, Hubert ; Nencka, Radim ; Dračínský, Martin
Document type: Papers
Conference/Event: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Year: 2014
Language: eng
Abstract: Phosphoramidate derivatives are often used as prodrugs of modified nucleosides. The crucial step for the biologically active compound release in cells is then enzymatic hydrolysis of the phosphoramidate derivative. The enzymatic decomposition of a nucleoside prodrug 1 was monitored using 31P NMR spectroscopy and the structure of the reaction products was determined by 1D and 2D (COSY, HSQC, HMBC) NMR experiments and compared with the products obtained with non-enzymatic hydrolysis of the prodrug.
Keywords: enzymatic hydrolysis; NMR spectroscopy; phosphoramidate derivatives
Project no.: GA13-24880S (CEP), GPP207/12/P625 (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234445

Permalink: http://www.nusl.cz/ntk/nusl-174774


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers