Original title: Preparation of modified oligonucleotides by nicking enzyme amplification reaction
Authors: Ménová, Petra ; Hocek, Michal
Document type: Papers
Conference/Event: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Year: 2014
Language: eng
Abstract: A method for the enzymatic synthesis of short (10-22 nt) base-modified oligonucleotides was developed, based on the nicking enzyme amplification reaction. The methodology, including product isolation and purification, was scaled up to nanomolar amounts. The modified oligonucleotides were successfully used as primers in primer extension and PCR, affording primer-modified oligonucleotides and DNA. Two simple and efficient methods for fluorescent labelling of the PCR products were also developed.
Keywords: DNA; dNTPs; oligonucleotides
Project no.: GBP206/12/G151 (CEP), GA203/09/0317 (CEP), IAA400040901 (CEP)
Funding provider: GA ČR, GA ČR, GA AV ČR
Host item entry: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234438

Permalink: http://www.nusl.cz/ntk/nusl-174768


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers