Original title: Vinyl-modified DNA and its cleavage by restriction endonucleases
Authors: Mačková, Michaela ; Hocek, Michal
Document type: Papers
Conference/Event: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Year: 2014
Language: eng
Abstract: Three types of vinyl modified nucleoside triphosphates (dNVTPs) were prepared by single step aqueous Suzuki- Miyaura cross-coupling reaction of halogenated 2’-deoxynucleotides with vinyltrifluoroborate. These dNVTPs were enzymatically incorporated into DNA in Primer extension experiments and Polymerase chain reaction. Primer extension products were then tested for cleavage by series of type II restriction enzymes and the results were compared with a cleavage of ethynyl-modified DNA.
Keywords: cross-coupling reaction; DNA; dNVTPs
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234437

Permalink: http://www.nusl.cz/ntk/nusl-174767


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers