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Original title:
Flow cytometry (FCM) sperm assessment In normozoospermic and asthenozoospermic men using monoclonal antibodies against sperm proteins
Authors: Čapková, Jana ; Kubátová, Alena ; Děd, Lukáš ; Teplá, O. ; Pěknicová, Jana
Document type: Papers
Conference/Event: XXth Symposium of Biology and Immunology of Reproduction with international participation, Třešť (CZ), 2014-05-22 / 2014-05-24
Year: 2014
Language: eng
Abstract: Recent studies have shown that infertility affects an estimated 15% of all couples. Male infertility is the primary or contributing cause in 60% of these cases. Consequently, application of methods of assisted reproduction is increasing. These methods would benefit from extended evaluation of the sperm quality. For this purpose, we analyzed sperm proteins in men with normal spermiograms and with asthenozoospermia. Ejaculates of both groups were tested with a set of well-characterized monoclonal antibodies (MoAbs) to human sperm. No statistically significant differences were found between normospermics and asthenospermics in the expression of sperm surface proteins clusterin, evaluated by Hs-3 MoAb, and semenogelin, evaluated by Hs-9 MoAb. On the other hand, flow cytometry revealed quantitative differences between normozoospermic and asthenozoospermic men in GAPDHS (glyceraldehyde phosphate dehydrogenase human sperm-specific glycolytic enzyme), evaluated by Hs-8 MoAb, VCP (valosin-containing protein), detected with Hs-14 MoAb, and PRKAR2A (cAMP-dependent protein kinase type II – alpha regulatory subunit) detected by MoAb Hs-36. Asthenozoospermic men displayed significantly reduced expression of intra-acrosomal proteins with a likely decrease in sperm quality, and thus a negative impact on successful reproduction.
Keywords: asthenozoospermia; flow cytometry; monoclonal antibody; spermatozoa
Project no.: CEZ:AV0Z50520701 (CEP), P503/12/1834, CZ1.05/1.1.00/02.0109
Funding provider: GA ČR, GA MŠk
Host item entry: Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation
Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234004
Permalink: http://www.nusl.cz/ntk/nusl-173876
The record appears in these collections:
Research > Institutes ASCR > Institute of Biotechnology
Conference materials > Papers
Authors: Čapková, Jana ; Kubátová, Alena ; Děd, Lukáš ; Teplá, O. ; Pěknicová, Jana
Document type: Papers
Conference/Event: XXth Symposium of Biology and Immunology of Reproduction with international participation, Třešť (CZ), 2014-05-22 / 2014-05-24
Year: 2014
Language: eng
Abstract: Recent studies have shown that infertility affects an estimated 15% of all couples. Male infertility is the primary or contributing cause in 60% of these cases. Consequently, application of methods of assisted reproduction is increasing. These methods would benefit from extended evaluation of the sperm quality. For this purpose, we analyzed sperm proteins in men with normal spermiograms and with asthenozoospermia. Ejaculates of both groups were tested with a set of well-characterized monoclonal antibodies (MoAbs) to human sperm. No statistically significant differences were found between normospermics and asthenospermics in the expression of sperm surface proteins clusterin, evaluated by Hs-3 MoAb, and semenogelin, evaluated by Hs-9 MoAb. On the other hand, flow cytometry revealed quantitative differences between normozoospermic and asthenozoospermic men in GAPDHS (glyceraldehyde phosphate dehydrogenase human sperm-specific glycolytic enzyme), evaluated by Hs-8 MoAb, VCP (valosin-containing protein), detected with Hs-14 MoAb, and PRKAR2A (cAMP-dependent protein kinase type II – alpha regulatory subunit) detected by MoAb Hs-36. Asthenozoospermic men displayed significantly reduced expression of intra-acrosomal proteins with a likely decrease in sperm quality, and thus a negative impact on successful reproduction.
Keywords: asthenozoospermia; flow cytometry; monoclonal antibody; spermatozoa
Project no.: CEZ:AV0Z50520701 (CEP), P503/12/1834, CZ1.05/1.1.00/02.0109
Funding provider: GA ČR, GA MŠk
Host item entry: Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation
Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0234004
Permalink: http://www.nusl.cz/ntk/nusl-173876
The record appears in these collections:
Research > Institutes ASCR > Institute of Biotechnology
Conference materials > Papers
Record created 2014-06-19, last modified 2021-11-24