Original title: IEF and HPLC-based methods for efficient bacterial characterization
Authors: Kubesová, Anna ; Šalplachta, Jiří ; Moravcová, Dana ; Horká, Marie
Document type: Papers
Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year: 2013
Language: eng
Abstract: Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi was described and it becomes serious problem for potato production in Europe. In this study, we suggest procedures using two commonly available laboratory techniques, gel isoelectric focusing and liquid chromatography, for differentiation and characterization of Pectobacterium and Dickeya species. We have confirmed that fingerprinting approach can be used for bacterial differentiation, which can be further used in rapid diagnosis of plant disease.
Keywords: Dickeya species; gel isoelectric focusing; liquid chromatography; Pectobacterium
Project no.: EE2.3.20.0182, VG20102015023 (CEP), VG20112015021 (CEP)
Funding provider: GA MŠk, GA MV, GA MV
Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0228934

Permalink: http://www.nusl.cz/ntk/nusl-170558


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Conference materials > Papers