Original title:
Porous layer open tubular columns with immobilized trypsin for protein digestion
Authors:
Knob, R. ; Křenková, Jana ; Petr, J. ; Foret, František Document type: Papers Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year:
2013
Language:
eng Abstract:
We have developed a monolithic porous layer open tubular (PLOT) column with immobilized trypsin for protein digestion. The PLOT column was prepared in a 10 (mikro)m ID fused silica capillary. Trypsin was immobilized on the monolithic surface and the developed enzyme reactor was used for protein digestion followed by on-line ESI/MS analysis.
Keywords:
digestion; enzymes; monolith Project no.: EE2.3.20.0182, M200311201 Funding provider: GA MŠk, GA AV ČR Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770
Institution: Institute of Analytical Chemistry AS ČR
(web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0228928