Original title: Porous layer open tubular columns with immobilized trypsin for protein digestion
Authors: Knob, R. ; Křenková, Jana ; Petr, J. ; Foret, František
Document type: Papers
Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year: 2013
Language: eng
Abstract: We have developed a monolithic porous layer open tubular (PLOT) column with immobilized trypsin for protein digestion. The PLOT column was prepared in a 10 (mikro)m ID fused silica capillary. Trypsin was immobilized on the monolithic surface and the developed enzyme reactor was used for protein digestion followed by on-line ESI/MS analysis.
Keywords: digestion; enzymes; monolith
Project no.: EE2.3.20.0182, M200311201
Funding provider: GA MŠk, GA AV ČR
Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0228928

Permalink: http://www.nusl.cz/ntk/nusl-170557


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers