Název:
Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases
Překlad názvu:
Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases
Autoři:
SAJWAN, Suresh Chandra Singh Typ dokumentu: Disertační práce
Rok:
2013
Jazyk:
cze
Abstrakt: This thesis describes the establishment of a precise gene targeting methodology in the silkworm Bombyx mori by technologies based on engineered endonucleases. Two classes of engineered endonucleases, ZFNs and full length TALENs were used for creating DSBs at specified sites in the colour marker genes (BmBlos2 and Bmwh3). Direct embryo microinjection of engineered nucleases mRNA were performed and let the nuclease proteins to disrupt the functions of these marker genes by creating DSBs and inducing error prone NHEJ mechanism. These experiments showed that both ZFNs and TALENs could be used for targeted gene disruption in silkworms.
Klíčová slova:
Bm1 element; BmBLOS2; Chimeric nucleases; Gene targeting; Lepidoptera; MMEJ; Nonhomologous end joining; ZFN; Bm1 element; BmBLOS2; Chimeric nucleases; Gene targeting; Lepidoptera; MMEJ; Nonhomologous end joining; ZFN Citace: SAJWAN, Suresh Chandra Singh. Gene targeting in Silkworm (Bombyx mori) by Engineered Endonucleases. České Budějovice, 2013. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Instituce: Jihočeská univerzita v Českých Budějovicích
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Informace o dostupnosti dokumentu:
Plný text je dostupný v digitálním repozitáři JČU. Původní záznam: http://www.jcu.cz/vskp/13951