Original title: Separation of clinically significant microorganisms from CSF by CIEF with UV detection and MALDI-TOF MS
Authors: Horká, Marie ; Šalplachta, Jiří ; Kubesová, Anna ; Růžička, F. ; Šlais, Karel
Document type: Papers
Conference/Event: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Year: 2012
Language: eng
Abstract: The identification of most of the clinically significant species by the conventional laboratory methods is still time-consuming and often insufficient for ensuring early targeted therapy. This is especially important in the case of the nosocomial infections where the number of microorganisms in the cerebrospinal fluid is often ranging from units up to hundreds of cells per milliliter during bacterial infections of the central nervous system. The electrophoretic techniques with UV-detection reach the detection limit of intact cells approximately from 107 cells mL-1.
Keywords: capillary isoelectric focusing; labeled microorganisms; pre-concentration
Project no.: VG20102015023 (CEP)
Funding provider: GA MV
Host item entry: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3
Note: Související webová stránka: http://hdl.handle.net/11104/0215758

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0215758

Permalink: http://www.nusl.cz/ntk/nusl-135536


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