Original title: Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice
Authors: Vykydalová, Marie ; Horká, Marie ; Šalplachta, Jiří ; Růžička, E. ; Kahle, Vladislav
Document type: Papers
Conference/Event: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Year: 2012
Language: eng
Abstract: In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories.
Keywords: biofilm; Candida; fingerprint
Project no.: VG20112015021 (CEP), EE2.3.20.0182
Funding provider: GA MV, GA MŠk
Host item entry: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3
Note: Související webová stránka: http://hdl.handle.net/11104/0215227

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0215227

Permalink: http://www.nusl.cz/ntk/nusl-135524


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