Original title: The efficient approaches for enzymatic digestion of plant proteins
Authors: Dyčka, Filip ; Bobáľ, P. ; Bobálová, Janette
Document type: Papers
Conference/Event: CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./, Brno (CZ), 2012-11-01 / 2012-11-02
Year: 2012
Language: eng
Abstract: In this work the fast and efficient methods applying ultrasound and infrared radiation were employed for in-gel digestion of proteins extracted from Hordeum vulgare. These techniques can be used instead of time consuming conventional protocol prior mass spectrometry analysis. The comparative study indicates that the examined protocols are able to enhance digestion efficiency and protein identification.
Keywords: digestion; infrared; ultrasonic
Project no.: 1M0570 (CEP), 1M06030 (CEP), EE2.3.20.0182
Funding provider: GA MŠk, GA MŠk, GA MŠk
Host item entry: CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-1-3
Note: Související webová stránka: http://hdl.handle.net/11104/0215222

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0215222

Permalink: http://www.nusl.cz/ntk/nusl-135523


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers