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Original title:
(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer
Authors: Josypčuk, Bohdan ; Mareček, Vladimír ; Yosypchuk, O.
Document type: Papers
Conference/Event: Moderní elektrochemické metody /32./, Jetřichovice (CZ), 2012-05-21 / 2012-05-25
Year: 2012
Language: eng
Abstract: Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 μg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor.
Keywords: amalgam electrodes; monolayer; voltammetry
Project no.: GAP206/11/1638 (CEP), GAP208/12/1645 (CEP), IAA400400806 (CEP)
Funding provider: GA ČR, GA ČR, GA AV ČR
Host item entry: XXXII. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-0-7
Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0007008
Permalink: http://www.nusl.cz/ntk/nusl-113896
The record appears in these collections:
Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers
Authors: Josypčuk, Bohdan ; Mareček, Vladimír ; Yosypchuk, O.
Document type: Papers
Conference/Event: Moderní elektrochemické metody /32./, Jetřichovice (CZ), 2012-05-21 / 2012-05-25
Year: 2012
Language: eng
Abstract: Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 μg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor.
Keywords: amalgam electrodes; monolayer; voltammetry
Project no.: GAP206/11/1638 (CEP), GAP208/12/1645 (CEP), IAA400400806 (CEP)
Funding provider: GA ČR, GA ČR, GA AV ČR
Host item entry: XXXII. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-0-7
Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0007008
Permalink: http://www.nusl.cz/ntk/nusl-113896
The record appears in these collections:
Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers
Record created 2012-06-18, last modified 2024-01-26