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OCT activity and glycation damage in rat liver mitochondria
Merhautová, Lucie ; Dršata, Jaroslav (advisor) ; Boušová, Iva (referee)
OCT ACTIVITY AND GLYCATION DAMAGE IN RAT LIVER MITOCHONDRIA Lucie Merhutová Diploma thesis. Charles University in Prague, Faculty of Pharmacy in Hradec Králové. 2007 This work was created following investigations carried out in LBBCV (Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement) at University Paris Diderot - Paris 7. The team of this laboratory studies the mechanisms of posttranslational non-enzymatic modifications of proteins, involved in the pathophysiology of aging. Glycation is one of the mechanisms responsible for the modification of intracellular macromolecules leading to the loss of their structure and function. In previous investigations, ornithine carbamoyltransferase (OCT), the second enzyme of urea cycle, has been identified as one of the markedly glycated proteins in liver mitochondria of senescent rats. The goal of this work was to investigate in vitro and ex vivo glycation of OCT and the effect of glycation on its function. Purified OCT and mitochondrial matrix extracts were incubated with methylglyoxal, one of the most reactive physiological glycating agents, and submitted to the enzymatic assay and immunochemical analysis. We demonstrated that methylglyoxal modifies OCT both in vitro and ex vivo and causes rapid and extensive decrease in its enzymatic activity....
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Elsa Goller and Julius Zeyer: friendship between translator and author
Friedlová, Maria ; Topor, Michal (advisor) ; Merhautová, Lucie (referee)
(in English): This bachelor's thesis aims to provide an introduction of Elsa Goller regarding her relationship with the Czech poet Julius Zeyer. The thesis is primarily based on their extensive correspondence deposited in the Literary Archive of the Museum of Czech Literature; another helpful source of information is represented by a thorough and detailed file including not only cuttings of Goller's texts (such as articles and translations), but also historical texts about her. In the thesis, special attention is devoted to the part of Goller's journalistic and translation work concentrated in the old Bohemian daily, the Politik (or the Union). In this very daily, most her translations of Zeyer's works were published. By Zeyer and Goller's multilingual correspondence, not only their long-lasting friendship, but also their translation cooperation is revealed. To provide a better understanding of their relationship, a wide look suggesting Zeyer's attitude towards women is taken, as well as the outline of the situation and social status of women translators towards the end of the 19th century is depicted.
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Czech Literary Anarchism in the Context of Socialism and the Women's Movement (1890-1914)
Hylmar, Radek ; Heczková, Libuše (advisor) ; Wiendl, Jan (referee) ; Merhautová, Lucie (referee)
Czech Literary Anarchism in the Context of Socialism and the Women's Movement (1890-1914) Abstract The thesis focuses on Czech pre-WWI anarchism. It analyses it as a modernist movement comprising various activities spanning political propaganda, proposals of social organisation and thinking about moral values as well views on the arts and literary production. The aim is to present literary texts written by anarchists against a backdrop of other types of expression. At the same time, the thesis assesses anarchism in the historical context of other political, social, artistic and philosophical movements. We study the interweaving of ideological and aesthetic schemes of Czech anarchism, especially with socialism and feminism, but also concerning decadence, Friedrich Nietzsche's philosophy and writings of the German Friedrichshagen poets' and anarchists' circle. The thesis focuses on how anarchism understands human beings and their relationship to the world and society. Given the anarchists' focus on the free individual, we present strategies of emancipation from traditional conventions and institutions such as marriage, family and the state. We also concentrate on reforms concerning morals and similarities with the feminist turn to one's own bodily and psychical experiences as starting points for setting...
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OCT activity and glycation damage in rat liver mitochondria
Merhautová, Lucie ; Dršata, Jaroslav (advisor) ; Boušová, Iva (referee)
OCT ACTIVITY AND GLYCATION DAMAGE IN RAT LIVER MITOCHONDRIA Lucie Merhutová Diploma thesis. Charles University in Prague, Faculty of Pharmacy in Hradec Králové. 2007 This work was created following investigations carried out in LBBCV (Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement) at University Paris Diderot - Paris 7. The team of this laboratory studies the mechanisms of posttranslational non-enzymatic modifications of proteins, involved in the pathophysiology of aging. Glycation is one of the mechanisms responsible for the modification of intracellular macromolecules leading to the loss of their structure and function. In previous investigations, ornithine carbamoyltransferase (OCT), the second enzyme of urea cycle, has been identified as one of the markedly glycated proteins in liver mitochondria of senescent rats. The goal of this work was to investigate in vitro and ex vivo glycation of OCT and the effect of glycation on its function. Purified OCT and mitochondrial matrix extracts were incubated with methylglyoxal, one of the most reactive physiological glycating agents, and submitted to the enzymatic assay and immunochemical analysis. We demonstrated that methylglyoxal modifies OCT both in vitro and ex vivo and causes rapid and extensive decrease in its enzymatic activity....
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