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Optimization of the HPLC method for determination of propranolol in liquid oral formulations
Koval, Tomáš ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tomáš Koval Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Optimization of the HPLC method for determination of propranolol in liquid oral formulations The purpose of thesis is the optimization and subsequent validation of HPLC method for determination of propranolol - hydrochloride in liquid oral formulations. A medicinal product containing propranolol - hydrochloride is used in the therapy of infantile haemangiomas as extemporaneous prepared solution. For method optimization were chosen Kinetex® C18 column (150x4,6 mm, size of particles 5 μm) and isocratic elution of mobile phase containing phosphate buffer (c=17,7 mM) and acetonitrile in 30:70 ratio, with pH adjusted to 2,2. Flow rate was set at 1,2 ml/min. According to results of the validation, was found that this developed method for the analysis of the product is suitable, and provides accurate and precise results. Keywords: propranolol hydrochloride, HPLC, optimization, Sodium benzoate, liquid oral formulations
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Optimization of the HPLC method for determination of propranolol in liquid oral formulations
Koval, Tomáš ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tomáš Koval Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Optimization of the HPLC method for determination of propranolol in liquid oral formulations The purpose of thesis is the optimization and subsequent validation of HPLC method for determination of propranolol - hydrochloride in liquid oral formulations. A medicinal product containing propranolol - hydrochloride is used in the therapy of infantile haemangiomas as extemporaneous prepared solution. For method optimization were chosen Kinetex® C18 column (150x4,6 mm, size of particles 5 μm) and isocratic elution of mobile phase containing phosphate buffer (c=17,7 mM) and acetonitrile in 30:70 ratio, with pH adjusted to 2,2. Flow rate was set at 1,2 ml/min. According to results of the validation, was found that this developed method for the analysis of the product is suitable, and provides accurate and precise results. Keywords: propranolol hydrochloride, HPLC, optimization, Sodium benzoate, liquid oral formulations
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Structure Analysis of Plant Bifunctional Nuclease TBN1
Kovaľ, Tomáš ; Dohnálek, Jan (advisor) ; Brynda, Jiří (referee) ; Schneider, Bohdan (referee)
This work is dedicated to thorough structural analysis of plant bifunctional nuclease TBN1, the representative of plant nuclease I group. TBN1 along with homologous nucleases from this family plays an important role in plant cell life cycle and also shows considerable anticancerogenic effects. Two variants of TBN1 (wild type and N211D mutant) were studied. Properties of both variants in different solutions were analyzed. Both were successfully crystallized. Structures of both types of TBN1 were solved using X-ray diffraction. The phase problem was solved by Multi-wavelength anomalous dispersion using Zn2+ ions natively present in TBN1. Structural properties of TBN1 such as fold, active site composition, effect of glycosylation and surface electrostatic potential distribution were analyzed. Reaction mechanism of TBN1 was proposed on the basis of structural properties and comparisons with similar structures.
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The theoretical exploration of the interactions of dirhodium complexes.
Kovaľ, Tomáš ; Burda, Jaroslav (advisor) ; Záliš, Stanislav (referee)
This work focuses on the study of interactions of the Diaqua-tetrakis(-acetylato)dirhodium(II,II) complex. This complex belongs to compounds of transition metals, which show activity in anticancer treatment. In this work, the interactions of this complex with ammonia and guanine are studied. The interactions with ammonia are represented by a replacement reaction of oxygen from acetyl ligand or aqua ligand. Another reaction is the replacement of water bonded to rhodium by guanine. Guanine can form a bond to rhodium with oxygen O6 or nitrogen N7. Optimization of the structures are made at the DFT level with 6-31G* basis set. For reactions with ammonia transition states were found. NPA charge analyses, thermodynamical and kinetical analyses were performed. All these analyses were done at the DFT level with aug-cc-pvdz basis set. To describe the electronic structure of rhodium atoms pseudopotentials and pseudobasis were used. All calculations were modeled in gas phase.
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