|
|
Formulation of vancomycin loaded PLGA nanoparticles for local treatment of musculoskeletal infections II.
Ptáčková, Karolína ; Šnejdrová, Eva (advisor) ; Zbytovská, Jarmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Karolína Ptáčková Title of thesis: Formulation of vancomycin loaded PLGA nanoparticles for local treatment of musculoskeletal infections II. Supervisor: PharmDr. Eva Šnejdrová, Ph.D. Consultant: Mgr. Vladislav Frolov The aim of the work was to formulate vancomycin hydrochloride (Van-HCl) loaded PLGA nanoparticles (NPs) using a double emulsion method for the local treatment of musculoskeletal infections. During preparation, selected critical formulation factors were modified and their influence on the characteristics of nanoparticles was studied. The size of the NPs determined by the DLS method ranged from 200 to 800 nm, the PDI was lower than 0.2 in most batches. The encapsulation efficiency (EE) determined by the direct method ranged from 2 % to 9 %. Glycerol monostearate at a concentration of 15 mg/ml proved to be a suitable primary emulsion stabilizer. The optimal concentration of PVA for stabilizing the nanosuspension was 30 mg/ml. The size of the NPs was influenced by the intensity of homogenization and the concentration of PVA. The concentration of Van-HCl, the organic solvent used and the intensity of homogenization led to an increase in EE. The DSC method confirmed that Van-HCl remains...
|
|
|
Adhesive properties of thin layers based on plasticized polyesters
Soukupová, Jana ; Šnejdrová, Eva (advisor) ; Šklubalová, Zdeňka (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Name: Jana Soukupova Title of diploma thesis: Adhesive properties of thin layers based on plasticized polyesters Supervisor: PharmDr. Eva Snejdrova, Ph.D. This diploma thesis deals with rheological, adhesive and dissolution properties of thin layers based on the branched polyester of D,L- lactic acid and glycolic acid, plasticized with methyl- salicylate, and loaded with salicylic acid. The theoretical section describes bioadhesion, its mechanism and theories, in vitro adhesive tests focused mainly on the method of rheological synergism, tensile and "wash off" tests. In the experimental section, the flow properties of plasticized polyesters, hydrated mucin from porcin stomach, and their mixtures were studied using absolute rotational rheometer. The analysis of the viscosity curves revealed that plasticized polyesters are Newtonian systems, mucin and its mixtures with polymer have pseudoplastic behaviour. Adhesive properties of the thin layers were established by equation of rheological synergism based on viscosity of polymer, mucin and their mixtures measured at shear rate of 10 s-1 . The adhesive properties of thin layers were also determined using tensile test on rheometer, and evaluated as the...
|
|
|
Efect of the composition of an emulsion with imunosupressant on the rhelogical properities of the emulsion
Martinek, Vojtěch ; Šnejdrová, Eva (advisor) ; Vraníková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Mgr. Vojtěch Martinek Title of thesis: Efect of the composition of an emulsion with imunosupressant on the rhelogical properities of the emulsion Supervisor: PharmDr. Eva Snejdrova, Ph.D. This thesis evaluates rheological properties of the emulsion created after mixing the drug product Cyclosporine Oral Solution, USP /MODIFIED/ 100 mg/ml with water in different ratios. The theoretical part describes general characteristics, stability and viscosity of emulsions. It also describes self-emulsifying systems and the tested product Cyclosporine Oral Solution, USP /MODIFIED/ 100 mg/ml. In the experimental part of the work, the type of emulsion created, its flow properties and the effect of homogenization intensity were characterized. It was experimentally verified that as the amount of water in the mixture increases, first a w/o emulsion is formed and then the emulsion turns to an o/w emulsion type. In terms of flow properties, the medicinal product exhibits Newtonian behavior. By gradually adding water, the behavior changes to non-Newtonian up to a water:product ratio of 70:30, where the minimum value of the flow behavior index is found. As water is further added, the properties change back to...
|
|
|
Adhesivity of in situ PLGA films for the local drug delivery.
Paňkiv, Julie ; Šnejdrová, Eva (advisor) ; Mužíková, Jitka (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Mentor: PharmDr. Eva Šnejdrová, Ph.D. Consultant: PharmDr. Věříš Andrea Student: Julie Paňkiv Title of Thesis: Adhesivity of in situ PLGA films for the local drug delivery The goal of this work was the formulation and study of the film forming systems FFS formed by poly(lactic-co-glycolic) acid of linear or branched structure, plasticized with methyl- salicylate, ethyl-pyruvate or ectoine, with incorporated cannabidiol. Acetone and ethyl acetate were tested as solvents. The theoretical part is focused on the characterization of FFS, their composition and testing. In situ films were characterized by DSC and SEM. The course of evaporation of the organic solvent from the FFS was monitored. A methodology for testing adhesion of in situ films using a tensile test on a rheometer was developed. It was found that the evaporation time of the organic solvent from FFS is influenced by both the type of solvent used and the type of plasticizer. In non-plasticized polymers, organic solvent evaporates faster than in plasticized ones. Evaporation of more than 90 % of the solvent occurs within 5 minutes. Compared to the commercial preparation Lamisil Once, the tested PLGA in situ films show higher adhesiveness....
|
|
|
Adhesivity of in situ PLGA films for the local drug delivery.
Paňkiv, Julie ; Šnejdrová, Eva (advisor) ; Mužíková, Jitka (referee)
Charles University, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical technology Mentor: PharmDr. Eva Šnejdrová, Ph.D. Consultant: PharmDr. Věříš Andrea Student: Julie Paňkiv Title of Thesis: Adhesivity of in situ PLGA films for the local drug delivery The goal of this work was the formulation and study of the film forming systems FFS formed by poly(lactic-co-glycolic) acid of linear or branched structure, plasticized with methyl- salicylate, ethyl-pyruvate or ectoine, with incorporated cannabidiol. Acetone and ethyl acetate were tested as solvents. The theoretical part is focused on the characterization of FFS, their composition and testing. In situ films were characterized by DSC and SEM. The course of evaporation of the organic solvent from the FFS was monitored. A methodology for testing adhesion of in situ films using a tensile test on a rheometer was developed. It was found that the evaporation time of the organic solvent from FFS is influenced by both the type of solvent used and the type of plasticizer. In non-plasticized polymers, organic solvent evaporates faster than in plasticized ones. Evaporation of more than 90 % of the solvent occurs within 5 minutes. Compared to the commercial preparation Lamisil Once, the tested PLGA in situ films show higher adhesiveness....
|
|
|
Formulation of vancomycin loaded PLGA nanoparticles for local treatment of musculoskeletal infections I.
Petrášová, Ivana ; Šnejdrová, Eva (advisor) ; Smékalová, Monika (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Ivana Petrášová Title of thesis: Formulation of PLGA nanoparticles with vancomycin for local treatment of musculoskeletal infections I Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The treatment of musculoskeletal infections requires the presence of high antibiotics concentration at the infection site. However, high doses of orally administered drugs cause the risk of increased systemic toxicity and serious adverse effects. Targeted drug delivery, currently one of the main areas of pharmaceutical research, represents a suitable option of a treatment without excessive burden on the organism. This diploma thesis focuses on the formulation of a therapeutic system for the local treatment of musculoskeletal infections in the form of polymer nanoparticles containing the antibiotic vancomycin. Due to the need of incorporation of a hydrophilic antimicrobial substance into them, the suspension-emulsion method of their preparation was used. Different concentrations of polyvinyl alcohol stabilizer and tripentaerythritol branched hydrophobic polymer poly(D,L- lactide-co-glycolide) were used for the preparation. Within the study the size of nanoparticles, polydispersity, zeta potential, encapsulation efficiency...
|
|
|
The preparation of tablets by selective laser sintering.
Ficek, Lukáš ; Mužíková, Jitka (advisor) ; Šnejdrová, Eva (referee)
Charles University, Faculty of pharmacy in Hradec Králové Department of: Pharmaceutical Technology Mentor: doc. PharmDr Jitka Mužíková, Ph. D Consultant: Mgr. Thao Tranová Student: Lukáš Ficek Title of Thesis: The preparation of tablets by laser sintering This work focuses on the preparation of orally dispersible tablets by selective laser sintering (SLS). The present project is a pilot study conducted on the 3D printer Sintratec Kit at the Department of Pharmaceutical Technology. SLS is a 3D printing method based on the fusion of powder particles using a laser beam that sinters the individual particles together in the layers of material.1-2 The matrix forming polymer used in work was Kollidon® VA64 which was combined with two pigments namely Candurin® Gold Sheen and NTX Ruby Red. In addition, mixtures of the polymer with a co-processed dry binder Prosolv® ODT G2 and a physical mixture of mannitol, silicified microcrystalline cellulose, and crospovidone at the concentrations of 20%, 40%, and 60% were used. The influence of printing parameters, especially laser speed, on tablet quality was tested. NTX Ruby Red pigment was selected for final tablet printing. The best powder formulation for optimal printing progress and high-quality properties of the orally dispersible tablets was the mixture of...
|
|
|
Formulation and characterization of w/o emulsions for local treatment of musculoskeletal infections
Vedralová, Štěpánka ; Šnejdrová, Eva (advisor) ; Mužíková, Jitka (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical technology Author: Štěpánka Vedralová Title of diploma thesis: Formulation and characterization of w/o emulsions for local treatment of musculoskeletal infections Supervisor: PharmDr. Eva Šnejdrová, Ph.D. The aim of the diploma thesis was to formulate a depot dosage form for the prevention and local treatment of musculoskeletal infections. A water-in-oil emulsions have been formulated which, due to the partition coefficient between the outer and inner phases, can provide sustained drug release. Oils with different viscosities were tested as oil phases. Tricaprin was chosen for the preparation of emulsions, due to the highest stability of the prepared emulsion. Lecithin and sorbitan monooleate or polyglyceryl-3-polyricinooleate (PGPR) and magnesium stearate were used as emulsifiers. The aqueous phase was a solution of vancomycin hydrochloride and gentamicin sulphate. The flow properties on a rotational rheometer were evaluated. The influence of the emulsion composition and the homogenization method on the coefficient of consistency and the index of flow behaviour of the emulsions was studied. The emulsions stabilized with lecithin have a higher viscosity and a structure more sensitive to changes in composition...
|
|
|
Formulation and characterization of hydrogels for local treatment of musculoskeletal infections
Drdová, Pavlína ; Šnejdrová, Eva (advisor) ; Vraníková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Pharmaceutical Technology Author: Pavlína Drdová Title of thesis: Formulation and characterization of hydrogels for local treatement of musculoskeletal infection Supervisor: PharmDr. Eva Šnejdrová, Ph.D. Local anti-infective therapy for musculoskeletal infections eliminates systemic drug toxicity. By selecting a suitable drug delivery system, higher drug concentrations at the site of infection and sustained release can be achieved. Such a system may be gels, formulated and tested in the submitted diploma thesis. The theoretical part of the work was devoted to the description of musculoskeletal infections and their treatment, characterization of gels and their use in parenteral administration. Hydrophilic gels based on hypromellose, carrageenan, gelatin and fish gelatin containing vamcomycin hydrochloride and gentamicin sulfate were formulated. Flow and viscoelastic properties were tested on a rotational rheometer. The analysis of flow curves by the Power law model proved a non-Newtonian type of flow. The most sensitive to applied stress is a 3 % hypromellose gel, the least a dispersion of iota-carrageenan with povidone 25. Viscoelastic properties were evaluated based on elastic, viscous and complex modulus, phase angle and yield...
|
|
|
Rheological properties of gelatin-based gels for ultrasound brain phantom
Bojňanská, Terézia Marianna ; Šnejdrová, Eva (advisor) ; Holas, Ondřej (referee)
CHARLES UNIVERSITY IN PRAGUE FACULTY OF PHARMACY IN HRADEC KRALOVE DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY Author: Terezia Marianna Bojnanska Supervisor: PharmDr. Eva Snejdrova, Ph.D. Title of diploma thesis: Rheological properties of gelatin-based gels for ultrasound brain phantom The diploma thesis deals with the evaluation of the viscoelastic properties of gelatin gels modified by addition of glutaraldehyde and glycerol using a rheometer with goal to verify the suitability of tested materials for their intended use as ultrasound brain phantoms. The theoretical part deals with gels in general and is focused on gelatin gels as a possible basic component of ultrasound phantoms, and the possibilities of characterizing gels as viscoelastic systems using methods of oscillatory rheometry. Gelatin gels of different concentrations (5; 7,5 and 10%) with the addition of glutaraldehyde and glycerol were characterized by complex modulus, complex viscosity, yield stress, phase angle. Temperature stability of the samples in the range from 20 to 50řC was tested. All tested gels were viscoelastic solids with a clearly defined linear-viscoelastic region, which is confirmed by phase angle values close to 0ř. With the increasing concentration of gelatin the values of the complex modulus increased, increasing...
|
guest ::
