National Repository of Grey Literature 59 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Tors and their microforms
Zemanová, Lucie ; Křížek, Marek (advisor) ; Vočadlová, Klára (referee)
This Bachelor thesis is focused on the general characteristics of tors and their microforms. There are two theories of tor's origin: one-stage origin and two-stage origin. Tors are developed all around the world, most often in temperate climatic zone. They show a wide range of sizes, shapes and locations. Tors are found on hill summits and on valley-sides. Tors occur on various lithologies, particularly on crystalline rocks. Many microforms can be found on the surface of tors. The most common microforms include weathering pits, tafoni, karren and honeycombs. The stage of their development enables microforms to be utilised for relative dating. Key words: tors, microforms, deep weathering, slope retreat
basic characterization of human enzymes DHRS7B and DHRS7C
Tučková, Tereza ; Zemanová, Lucie (advisor) ; Matoušková, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Tereza Tučková Supervisor: RNDr. Lucie Zemanová, Ph.D. Title: Basic characterization of human enzymes DHRS7B and DHRS7C Human enzymes of the short-chain dehydrogenase/reductase (SDR) superfamily play an important role in wide range of biochemical pathways. They are involved in metabolism of lipids, saccharides, amino acids, steroid hormones, retinoids, prostaglandins etc. Besides physiological processes, they take part in development of several serious diseases, e.g. hormone-dependent cancer, metabolic syndrome, diabetes mellitus. Moreover, SDR enzymes contribute to biotransformation of xenobiotics. Nevertheless, approximately 30 % of SDRs remain completely uncharacterized. Human dehydrogenase/reductase SDR family members 7B (DHRS7B) and 7C (DHRS7C) belong to poorly characterized members of the SDR superfamily. According to in silico predictions, both enzymes are membrane bound and involved in reductive reactions. The aim of this study was to determine their basic biochemical properties. The results show that both enzymes interact with the membrane of endoplasmic reticulum and face cytosol. The pilot screening of enzymatic activity was performed. Reducing activity was detected towards e.g....
Oxidative damage to proteins
Bártová, Jana ; Boušová, Iva (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Jana Bártová Supervisor: PharmDr. Iva Boušová, Ph.D. Title of diploma thesis: Oxidative damage to proteins Hypochlorous acid is a strong oxidant, which is used by phagocytic cells to kill ingested bacteria. Surrounding structures including proteins may be damaged in case the content of a phagocyte is spilled to the vicinity. Catechins belong to a group of flavonoids whose antioxidant properties were demonstrated in many studies. My diploma thesis was focused on a potential effect of eight naturally occurring derivatives of catechin towards oxidative damage of human serum albumin (HSA) by hypochlorous acid. I used two spectrophotometric (determination of carbonyl groups and chloramines) and three electrophoretic methods (native PAGE, SDS-PAGE, immunoblotting). Dependence of an antioxidant effect on its concentration (0-3 mM) was observed during determination of chloramines and obtained data were used for calculation of EC50 values of individual catechins using program GraphPad Prism. The most effective compound was catechin whose EC50 was 4.8 µM. Results suggest that antioxidant ability decreases with increasing number of hydroxyl groups in the molecule of flavonoid. Carbonyl groups are a...
Bussiness Plan
Zemanová, Lucie ; Pernica, Martin (referee) ; Hanušová, Helena (advisor)
The subject of this diploma thesis is the compilation of the business plan for the extension of the services of the Toy Shop * Stationery. This work consists of three main parts. The theoretical part contains definitions of terms forming the structure of the business plan and the definition of necessary analyzes. In the analytical part is analyzed the current state of the company. In the last proposed section is created a concrete business plan, which is aimed at the development of the company.
Optimization of procedure for isolation of phosphorylated peptides from a peptide mixture by metal oxide affinity chromatography for mass spectrometry analysis
Popovská, Lenka ; Solich, Petr (advisor) ; Zemanová, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Lenka Popovská Supervisor: Prof. RNDr. Petr Solich, CSc. Consultant Supervisor: Mgr. Barbora Šalovská, Ph.D. Title of the rigorous thesis: Optimization of procedure for isolation of phosphorylated peptides from a peptide mixture by metal oxide affinity chromatography for mass spectrometry analysis. Protein phosphorylation is the key regulatory mechanism in cellular signalling. It is involved in most cellular processes that are regulated by a complex interplay of protein kinases and protein phosphatases and include proliferation, differentiation, and apoptosis. Defective or altered signalling pathways can lead to various diseases, and therefore it is very important to study protein phosphorylation in detail and try to understand it as much as possible. Technological development of mass spectrometry (MS) in recent years has enabled a significant increase in the number of phosphorylation sites that may be identified in a sample. Increasing scan speed together with commercial availability of high resolution MS have improved quantity and quality of the acquired data, which can be obtained in a relatively short time. However, even high-end devices would not allow to comprehensively identify the...
basic characterization of human enzymes DHRS7B and DHRS7C
Tučková, Tereza ; Zemanová, Lucie (advisor) ; Matoušková, Petra (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Tereza Tučková Supervisor: RNDr. Lucie Zemanová, Ph.D. Title: Basic characterization of human enzymes DHRS7B and DHRS7C Human enzymes of the short-chain dehydrogenase/reductase (SDR) superfamily play an important role in wide range of biochemical pathways. They are involved in metabolism of lipids, saccharides, amino acids, steroid hormones, retinoids, prostaglandins etc. Besides physiological processes, they take part in development of several serious diseases, e.g. hormone-dependent cancer, metabolic syndrome, diabetes mellitus. Moreover, SDR enzymes contribute to biotransformation of xenobiotics. Nevertheless, approximately 30 % of SDRs remain completely uncharacterized. Human dehydrogenase/reductase SDR family members 7B (DHRS7B) and 7C (DHRS7C) belong to poorly characterized members of the SDR superfamily. According to in silico predictions, both enzymes are membrane bound and involved in reductive reactions. The aim of this study was to determine their basic biochemical properties. The results show that both enzymes interact with the membrane of endoplasmic reticulum and face cytosol. The pilot screening of enzymatic activity was performed. Reducing activity was detected towards e.g....
The group of DHRS enzymes and their role in human organism
Zamazalová, Alžběta ; Zemanová, Lucie (advisor) ; Štambergová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Alžběta Zamazalová Supervisor: RNDr. Lucie Zemanová, Ph.D. Title of diploma thesis: The group of DHRS enzymes and their role in human organism Human enzymes from dehydrogenase/reductase SDR family (DHRS) include 17 members that belong to the large short-chain dehydrogenase/reductase superfamily. The short-chain dehydrogenase/reductase superfamily contains 80 human members, some of them are important and well described enzymes such as 11β-hydroxysteroid dehydrogenase, carbonyl reductase 1 and 17β-hydroxysteroid dehydrogenase. However there are many poorly described or undescribed members of this superfamily, including group DHRS. There is just a little information about proteins DHRS1, DHRS12, DHRS13, DHRS4L1, DHRS4L2. Proteins DHRS2, DHRS4, DHRS6, DHRS7, DHRS7B, DHRS7C, DHRS8, DHRS9, DHRS10, DHRS11 and DHRSX are better described because at least subcellular localization and substrate (except DHRSX) is known, and therefore an initial opinion on their possible function can be formed. The DHRS3 is the only one enzyme of group DHRS that has been tested in vivo. DHRS3 could play a significant role in fetal development. For DHRS3, but also for the other DHRS enzymes, first knowledge of...
Effect of monepantel on expression of biotransformation enzymes in sheep
Štolcová, Tereza ; Skálová, Lenka (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Tereza Štolcová Supervisor:prof. RNDr. Lenka Skálová Ph.D. Title of diploma thesis: Effect of monepantel on expression of biotransformation enzymes in sheep Monepantel (MOP) represents a new anthelmintic drug from the amino-acetonitrile derivatives group with the different mode of action in comparison with commonly used broad-spectrum drugs. It is used for the treatment and control of gastrointestinal nematode infection in sheep and cattle. This study focuses on the effect of MOP on expression of selected cytochromes P450 (CYP) in sheep in vivo and in vitro. The sheep treated with the recommended therapeutic dose of MOP was used for the in vivo experiment. Non-treated animals represented the control group. Changes of mRNA expression of selected CYPs in primary cultures of ovine hepatocytes were observed after 24 h incubation with MOP (10 μM) in the in vitro experiment. The qPCR method (real time quantitative PCR) was used for the quantification of transcription levels of mRNA. Both in vivo as well as in vitro results showed a significant up-regulation in CYP3A24 expression. The induction effect of MOP was even stronger than induction effect of well-known CYP3A inducer rifampicin....

National Repository of Grey Literature : 59 records found   1 - 10nextend  jump to record:
See also: similar author names
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2 ZEMANOVÁ, Libuše
16 ZEMANOVÁ, Lucie
1 Zemanová, L.
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1 Zemanová, Ludmila
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