National Repository of Grey Literature 101 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Usage of HPLC and UHPLC-MS/MS techniques for clinical research
Kučerová, Kateřina ; Matysová, Ludmila (advisor) ; Doležal, Rafael (referee) ; Kučera, Radim (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Kučerová Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Consultant: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of Dissertation Thesis: Usage of HPLC and UHPLC - MS/MS techniques for clinical research The dissertation thesis is dealing with the extraction techniques and with development, optimization and validation of chromatographic methods for the determination of omeprazol which is drug from the group of proton pump inhibitors, and retinol as a new potential biomarker of kidney damage. The theoretical part of the thesis is focused on detailed description of the individual analytes, their clinical significance, options of determination and the matrix in which the target analytes were determined. Furthermore, the preparation of the sample before the analysis is discussed and validation recommendations related to chromatographic methods used in both pharmaceutical analysis and bioanalysis are also mentioned. In the experimental part the development, optimization and validation of UHPLC methods and their subsequent use are described in detail. The pharmaceutical-analytical part of the work describes the determination of omeprazole in new suspension preparations,...
Comparison of methods for determination of amphetamine derivates in meconium
Vrbová, Romana ; Matysová, Ludmila (advisor) ; Šatínský, Dalibor (referee)
Department: Department of Analytical Chemistry Candidate: Romana Vrbová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Comparison of methods for determination of amphetamine derivates in meconium The aim of the bachelor thesis was to optimize a new method for the determination of amphetamine derivatives in meconium, which will be faster and simpler than the existing routine method in the toxicological laboratory of Masaryk's Hospital in Ústí nad Labem. The theoretical part of the bachelor thesis describes the main representatives of amphetamine derivatives, their effects and metabolism. Their determination in meconium is important for the diagnosis of Neonatal abstinence syndrome, which occurs in newborns of mothers using addictive substances during pregnancy. Meconium, the first stool of a newborn, is a suitable material demonstrating exposure to drugs and medication during the second and third trimesters of pregnancy. Amphetamine derivatives were determined in meconium by gas chromatography with mass detection, the principles and instrumentalization of which are another part of the bachelor's thesis. The experimental part describes the optimization of a new, more advantageous method using pentafluorobenzoyl chloride as a derivatizing agent and its comparison with...
Stability testing of benzalconium chloride stock solution by HPLC
Křesťanová, Petra ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petra Křesťanová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Monitoring the stability of benzalkonium chloride stock solution by HPLC method This diploma thesis evaluates the stability of benzalkonium chloride in aqueous stock solution. The purpose of this work was to choose the most suitable conditions for storing and determine the shelf-life. This stability study was conducted by validated High Performance Liquid Chromatography method. Tested samples were prepared with pH 6.8 and 5 and stored in glass and plastic containers at room and lowered temperature in the absence of light. The stability was analysed in intervals of 0, 7, 14, 28, 60 and 90 days. All samples showed a good stability during the whole time of testing, without any significant differences. The recommended conditions for storing benzalkonium chloride solutions are room temperature and pH 6.8 in dark glass containers. These solutions can be used in the period of three months.
Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice
Kepka, Zdeněk ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Zdeněk Kepka Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of the diploma thesis: Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice This diploma thesis deals with the determination of neopterin and creatinine in urine samples by high performance liquid chromatography. The theoretical part summarizes information about the substances and their analysis. It also includes a review of methods published in the literature. The method of high-performance liquid chromatography including types of stationary phases and chromatographic principles used in the experimental part of this work is briefly introduced. The separation properties of six chromatographic columns for the determination of neopterin and creatinine in urine were verified in the experimental part of the thesis. Columns were compared by the following parameters: repeatability, resolution, peak symmetry, and column efficiency. The composition of the mobile phase was optimized for selected columns. The mobile phase was a phosphate buffer with a concentration of 15 mmol/l and pH 6.5 in most cases. Creatinine detection was performed by...
Development and validation of method for determination of of Cyclosporine in biorelevant mediums
Tichoň, Matej ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles university Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Matej Tichoň Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of method for determination of cyclosporine A in biorelevant mediums For the determination of cyclosporine A in biorelevant mediums, a new analytical method was developed by using of ultra-high performance liquid chromatography. The analysis was performed on a Kinetex™ 1.7 µm, C18 column, 50 x 2.1 mm (Phenomenex, USA). The mobile phase consisted of ultrapure water acidified with phosphoric acid to pH = 2.40 and mixture of methanol : acetonitrile (90 : 10) in a total ratio of 30 : 70 aqueous to organic phases. The analysis was carried out at 60 řC for 5 minutes with a constant flow rate of 0.5 ml/min. Gradient elution was used in the analysis, starting at 70 % organic phase at 0 minutes and increasing to 100 % at 5 minutes. Cyclosporine A was detected at 210 nm. The retention time of the substance was 3.08 min. Having the optimized conditions in hands, the method was fully validated. Keywords: cyclosporine A, UHPLC, biorelevant mediums
Monitoring of liberation tests.
Beran, Marek ; Sklenářová, Hana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department of Analytical Chemistry Candidate: Marek Beran Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Consultant: Ing. Daniela Šmejkalová, Ph.D Title of Diploma Thesis: Monitoring of liberation tests The diploma thesis is focused on the development of an automated method for the monitoring of the dissolution profile of clotrimazole from topical dosage forms. The dissolution profile of formulations containing the antifungal agent clotrimazole are compared. Commercially available formulations Canesten and Clotrimazol AL in the form of creams, are compared with a new formulation called Delcore from Contipro a.s., which contains a complex matrix. Theoretical part addresses mycotic infections with focus on superficial mycoses and products available on the Czech market for the treatment of those infections. Furthermore, there is a description of selected non-separation flow methods. The analysis was carried out in a sequential injection analysis system coupled with three attached Franz dissolution cells with a membrane on which the sample was applied. The Franz cells were placed in a water bath at 32 řC. Phosphate buffer with pH 7.4 was chosen as the dissolution medium as it partially imitates the conditions of the human environment. Detection...
Development of UHPLC method for determination of salicylic acid, methylsalicylate and ethylsalicylate in pharmaceutical formulations
Kosáčková, Nikola ; Matysová, Ludmila (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Nikola Kosáčková Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development of UHPLC method for determination of salicylic acid, methylsalicylate and ethylsalicylate in pharmaceutical formulations New chromatografic method for determination of salicylic acid, methylsalicylate and ethylsalicylate by using ultra-high performance liquid chromatography was developed. Analysis was carried out using the Kinetex® 1.7 µm XB-C18 100 Å, 50 x 2.1 mm column. The mobile phase was composed of aqueous solution of 1% acetic acid (pH 2.6) and methanol, and the flow rate was 0.4 ml/min. Separation was achieved using gradient elutions. An UV detector at the wavelength of 240 nm was used. Propylparaben was chosen as the internal standard. The total analysis time did not exceed 7 minutes. This method was used for the determination of releasing of salicylic acid and methylsalicylate from matrices consisting of polyester of D,L-lactic acid, glycolic acid and 8% dipentaerythritol (8D) and their dissolution media, which have been prepared at the Department of Pharmaceutical Technology at the FaF UK. Key words: Ultra-High Performance Liquid Chromatography, salicylic acid, methylsalicylate,...
Development and validation of UHPLC method for determination of miconazole, econazole and its impurities in solid dispersions
Arnoltová, Kristýna ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kristýna Arnoltová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Development and validation of UHPLC method for determination of miconazole, econazole and its impurities in solid dispersions The aim of the submitted thesis was to create a new analytical method for the determination of miconazole, econazole and its three impurities. Ultra-high performance liquid chromatography (UHPLC) was used. A KinetexTM XB C18 column, 1.7 μm particle size, 50 x 2.1 mm was used for the separation. The mobile phase was composed of the acetate buffer pH 7.8 in a mixture with methanol (80:20) and of acetonitrile with methanol (60:40), in a total ratio of 60:40. A gradient elution was used. Flow rate was set to 0.6 ml / min. Butylparaben was chosen as the internal standard. All substances were detected at a wavelength of 225nm. Total analysis time was 9 minutes. After optimization of the separation conditions, the method was partially validated and used for practical measurements to determine the content of miconazole in solid dispersions.
Stability testing of thiomersal stock solution by HPLC
Unverdorbenová, Veronika ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Veronika Unverdorbenová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Testing the stability of spare thiomersal solution by HPLC method. The aim of this thesis is testing the stability of thiomersal and subsequently establish conditions of storing the thiomersal solution. Reversed-Phase High Performance Liquid Chromatography was used for observing the concentrations of thiomersal in samples kept in different conditions. Tested samples were aqueous solutions of thiomersal with pH 6,85 and 7,5. These samples were stored in dark glass and plastic containers. Due to the non- photostability of thiomersal, the samples were stored in the dark. The temperature was laboratory and 4 řC. The samples were observed for the period of 90 days. Following the analysis, the most convenient conditions for storing the thiomersal solution is lowered temperature around 4 řC a pH 7,5. Under these storage conditions there were no substantial differences in the stability of thiomersal in the samples stored in plastic or glass containers. These samples were stable for the period of three months.

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