Institute of Molecular Genetics

Institute of Molecular Genetics 85 records found  previous11 - 20nextend  jump to record: Search took 0.00 seconds. 
Experimental methods for phase determination /MIR,MAD,SAD/ in crystallography of biological macromolecules
Brynda, Jiří
Importance of unbiased phases in post genomic era is emphasized. Article describes the classical methods of phase determination: 1) Multiple Isomorphous replacement, heavy-atom derivative (MIR) 2) Multiple Anomalous Dispersion (MAD) Phasing 3) Single Anomalous Dispersion (SAD) Phasing The better your initial, experimental map is, the more model you will be able to build confidently into this unbiased map before proceeding to phase combination. MAD phases result from perfectly isomorphous data, and do not deteriorate at low resolution as do MIR phases. MIR phases, on the other hand, provide superior phasing power at low resolution resulting in better connectivity. Not surprising, the best maps are either MAD or combined MIR-MAD maps.
Structural Studies of anti-CA IX Monoclonal Antibody M75 Fab Fragment in Complex with its Epitope Peptide
Mader, Pavel ; Štouračová, Renata ; Brynda, Jiří ; Závada, Jan
CA IX is a cell surface protein that exerts capacity of binding cell surface receptors, and is associated with several types of human carcinomas. Monoclonal antibody M75 recognizes specifically an epitope identified as six repeats of amino acid sequence PGEEDLP, localized in the proteoglycan like domain of CA IX. Structural study of the M75 Fab fragment in complex with its epitope peptide PGEEDLPGEEDL is presented.
Processing of Diffraction Data Obtained by Oscillation Method on Crystals of Biological Macromolecules
Brynda, Jiří
The components of monochromatic diffractometer suitable for data collection on crystals of biological macromolecules are described, as well as basics of rotation method. The optimal strategy for collecting X-ray diffraction data from macromolecular crystals is discussed. The geometric factors influencing the completeness of data are arising from the symmetry of the reciprocal lattice and from the experimental setup; they affect quantitatively the completeness of the measured set of reflections.
A Phenylnorstatine Inhibitor Binding to HIV-1 Protease: Geometry, Protonation and Subsite-Pocket Interactions Analyzed at Atomic Resolution
Brynda, Jiří ; Řezáčová, Pavlína ; Fábry, Milan ; Hořejší, Magdalena ; Štouračová, Renata ; Sedláček, Juraj ; Souček, M. ; Hradílek, M. ; Lepšík, M. ; Konvalinka, J.
The x-ray structure of a complex of HIV-1 protease (PR) with a phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH2 has been determined at 1.03 A, the highest resolution so far reported for any HIV PR complex. The inhibiot shows subnanomolar Ki values for both the wild-type PR and the variant representing one of the most common mutations linked to resistance developoment. The structure displays a unique pattern of hydrogen bonding to the two catalytic aspartate residues. The high resolution permits to assess the donor/acceptor realtions of this hydrogen bonding and to indicate a proton shared by the two catalytic residues. Structural mechanism for the unimpaired ihnibition of the protease Val82Ala mutant is also suggested, based on energy calculations and analyses.
Phytoremediation and biodegradation of PCB in soil
Ryšlavá, E. ; Krejčík, Zdeněk ; Macek, Tomáš ; Macková, M.
This is a part of the research work focused on study of possible mutual relationships of microorganisms and plants in PCBs metabolism. Polluted soil was obtained from a site which has been contaminated by PCBs for a long time. This soil was used for isolation of bacteria whixh potentially participate in biolodegradation of PCBs. The same contaminated soil was used for experiments under real conditions. Within six months three plant species were cultivated-tobaco (Nicotiana tabacum), black nighshade (Solanum nigrum) and alfalfa (Medicago sativa) - and decrease of PCBs was measured.
The regulation of antiinflammatory immune reactions in the frontal segment of the eye
Vítová, A. ; Filipec, M. ; Pindjáková, Jana ; Krulová, Magdalena ; Zajícová, Alena ; Holáň, Vladimír
Corneal stroma was identified as a source of a novel molecule rosponsible for selective ingibitor of production of anti-inflammatory cytokines.
Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J. ; Dvořáková, K. ; Pěknicová, Jana ; Kubátová, Alena ; Moore, H. D. M.
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes.
Dynamic and role of cytosekeletal proteins in mammalian sperm cells
Paleček, J. ; Dvořáková, K. ; Pěknicová, Jana ; Kubátová, Alena ; Dráber, Pavel
The cytoskeleton of sperm head consists of resistant structural proteins of the nucleus and of perinuclear theca, which are largely rosponsible for the shaping of the nucleus. three mahjor cyteskeletal proteins, actin, tzubnulin and spectrin, are present in the head of mammalian spermatohzoa. Maoreober, the mictotubular cytoskeleton also contains other dynamic proteins, which have a morphological function within the cell and which are responsible for cell signalling processes.
Study of sperm proteins in modelspecies of chondrostean and teleostean fish
Koubek, Pavel ; Elzeinová, Fatima ; Linhart, O. ; Pěknicová, Jana
Specific in the study of fish reproduction is outer fertilization. spermatozoa are usually expelled into host medium of the outer encironment. The cells have to cope with an extremely different encironment and their protection against the outer environment is much weaker than that of oocytes. The way of fertilization also differs from mammalian fertilization.
Regulation and localization of sperm akaps during capacitation, activation, induced acrosomal reaction and binding to the oocyte
Kyselová, Vendula ; Pěknicová, Jana
Recent evidence suggests that the cAMP-dependent protein kinase A (PKA) is localized to specific subcellulatr compatrments trought association with A Kinase Anchoring Proteins (AKAPs). The AKAPs now represent a functionally related family of regulatory proteins containing a conserved PKA binding domain and a unique targeting sequence that direct the PKA-AKAP complex to subcellular structures.

Institute of Molecular Genetics : 85 records found   previous11 - 20nextend  jump to record:
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