Original title: Využití pokročilých fluorescenčních metod ve studiu agregace
Translated title: Use of advanced fluorescence methods in the study of aggregation
Authors: Uhrínová, Alžbeta ; Enev, Vojtěch (referee) ; Venerová, Tereza (advisor)
Document type: Master’s theses
Year: 2024
Language: slo
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [slo] [eng]
Diplomová práca sa zaoberá použitím pokročilých fluorescenčných techník v štúdii agregácie vybraných agregačných modelov (kazeín a dodecylsulfát sodný). Na začiatku výskumu boli charakterizované fluorescenčné sondy Atto 488 a Rodamín 6G (RH6G) za použitia spektrofluorometrie, UV-VIS spektrometrie, časovo rozlíšenej fluorescencie a fluorescenčnej korelačnej spektroskopie (FCS). Pomocou rovnakých pokročilých fluorescenčných techník boli charakterizované agregačné modely s prídavkom fluorescenčných sond. Interakcia medzi fluorescenčnou sondou Atto 488 a agregačnými modelmi nebola potvrdená. Excitačné maximum Atto 488 bolo pri 500 nm a emisné maximum pri 520 nm. Doba života fluorescenčnej sondy Atto 488 bola od 4,20 do 4,82 ns a hydrodynamický polomer bol stanovený na 0,6 nm. Rodamín 6G mal excitačné maximum pri 525 nm a emisné maximum pri 555 nm. Doba života fluorescenčnej sondy RH6G bola od 3,99 do 5,27 ns. Zistilo sa, že intenzita RH6G po pridaní vyššej koncentrácie kazeínu klesá, čo má za následok zhášanie. Hydrodynamický polomer samotnej sondy RH6G bol stanovený na 0,6 nm. S prídavkom kazeínu pomocou vyhodnotenia metódou maximálnej entropie (MEM) bol od 0,5 do 534 nm. Stabilné micely SDS mali hydrodynamický polomer pomocou vyhodnotenia MEM 2,4 nm. The diploma thesis deals with the use of advanced fluorescence techniques in the study of aggregation of selected aggregation models (casein and sodium dodecyl sulfate). At the beginning of the research, fluorescent probes Atto 488 and Rhodamine 6G (RH6G) were characterized using a spectrofluorometry, UV-VIS spectrometry, time-resolved fluorescence, and fluorescence correlation spectroscopy (FCS). Aggregation models with the addition of fluorescent probes were characterized using the same advanced fluorescence techniques. The interaction between the fluorescent probe Atto 488 and the aggregation models was not confirmed. The excitation maximum of Atto 488 was at 500 nm and the emission maximum at 520 nm. The lifetime of the fluorescent probe Atto 488 was from 4,20 to 4,82 ns and the hydrodynamic radius was determined to 0,6 nm. Rhodamine 6G had an excitation maximum at 525 nm and an emission maximum at 555 nm. The lifetime of the fluorescent probe RH6G was from 3,99 to 5,27 ns. The intensity of RH6G was found to decrease upon addition of a higher concentration of casein, resulting in quenching. The hydrodynamic radius of the RH6G probe was determined to 0,6 nm. With the addition of casein using the maximum entropy method (MEM) evaluation was from 0,5 to 534 nm. Stable SDS micelles had a hydrodynamic radius by MEM evaluation of 2,4 nm.
Keywords: Atto 488; casein; Fluorescence; Rhodamine 6G; sodium dodecyl sulfate; spectroscopy

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: https://hdl.handle.net/11012/245708

Permalink: http://www.nusl.cz/ntk/nusl-615845


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Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Master’s theses