Název: A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity
Autoři: HAVLÍČKOVÁ, Petra
Typ dokumentu: Rigorózní práce
Rok: 2024
Jazyk: eng
Abstrakt: This RNDr. thesis is focused on complex studies of a haloacid dehalogenase superfamily phosphatase member with an unknown substrate specificity, isolated from a hyperthermofilic archeon Thermococcus thioreducens. The HAD phosphatase, internally named as Tt82, was isolated, expressed and purified using standard techniques and the crystal structure was solved by X-ray crystallography method. The refined crystal structure displayed characteristic HAD superfamily elements, such as the HAD canonical fold with the HAD signature motifs in the active site. Further, possible substrates for the enzyme were proposed and examined by computational docking in the active site of the HAD phosphatase. The results of the docking studies served as a basis for an experimental measurement of Tt82 enzymatic activity against selected substrates at two different temperatures. HAD phosphatase Tt82 is a promising target for the biotechnological field, mainly for its wide substrate specificity and higher temperature optima.
Citace: HAVLÍČKOVÁ, Petra. A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity. České Budějovice, 2024. rigorózní práce (RNDr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Instituce: Jihočeská univerzita v Českých Budějovicích (web)
Informace o dostupnosti dokumentu: Plný text je dostupný v digitálním repozitáři JČU.
Původní záznam: http://www.jcu.cz/vskp/74137

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-540621


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Školství > Veřejné vysoké školy > Jihočeská univerzita v Českých Budějovicích
Vysokoškolské kvalifikační práce > Rigorózní práce