Original title: Detailed Investigation of Human Galectins -1 and -3 by employing selectively deoxyfluorinated N-acetyllactosamines
Authors: Kurfiřt, Martin
Document type: Papers
Conference/Event: Bažant postraguate conference 2023, Prague (CZ), 20230531
Year: 2023
Language: eng
Abstract: This work is focused on a detailed investigation of supramolecular recognition events between human galectins -1 and -3 and monodeoxyfluorinated N-acetyllactosamine probes, which were previously prepared in our laboratory. The recognition was studied using a combination of X-ray crystallography, isothermal titration calorimetry and several advanced NMR techniques, such as 19F T2-filter, 1H–1H STD, 1H–15N CSP or 19F EXSY. These techniques provided insight into the thermodynamics and kinetics of recognition and enabled us to uncover of subtle differences between both investigated galectins. Such differences could be potentially exploited in the selective targeting of individual galectins with important implications for the galectins-focused\npharmaceutic industry.
Keywords: galectins; NMR techniques; pharmaceutic industry
Project no.: GA23-06115S (CEP), LTC20052, CA18103
Funding provider: GA ČR, GA MŠk
Host item entry: Proceedings of Abstracts, ISBN 978-80-86186-29-0
Rights: This work is protected under the Copyright Act No. 121/2000 Coll.

Institution: Institute of Chemical Process Fundamentals AS ČR (web)
Original record: https://hdl.handle.net/11104/0343242

Permalink: http://www.nusl.cz/ntk/nusl-526202


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