Original title: Syntéza a studium derivátů kyseliny hyaluronové modifikovaných na karboxylu
Translated title: Synthesis and study of hyaluronic acid derivatives modified on carboxyl
Authors: Volfová, Gabriela ; Roh, Jaroslav (advisor) ; Opálka, Lukáš (referee)
Document type: Rigorous theses
Year: 2023
Language: cze
Abstract: [cze] [eng]
Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra organické a bioorganické chemie Autor: Gabriela Volfová äkolitelé: Mgr. Radovan Bu a, Ph.D. doc. PharmDr. Jaroslav Roh, Ph.D. Název práce: Syntéza a studium derivát kyseliny hyaluronové modifikovan˝ch na karboxylu Abstrakt: Kyselina hyaluronová (HA) je p irozen se vyskytující lineární polysa- charid. Pro svou biokompatibilitu a neimunogenicitu jsou HA a její deriváty öiroce vyuûívány v r zn˝ch biologick˝ch a medicínsk˝ch aplikacích. P i formulaci konkrét- ního lé ivého p ípravku je pro jeho v˝slednou ú innost zásadní molekulová hmotnost (MW) HA, která vöak m ûe b˝t r zn˝mi vlivy v pr b hu v˝robního procesu ovliv- n na. V˝znamn˝m faktorem je zv˝öená teplota, nutná nap íklad p i sterilizaci pro- duktu, která vede ke sníûení v˝sledné MW polymeru. Tato práce se v nuje syntéze amid HA na její volné karboxylové skupin a následn studiu vlivu této modifikace na degradaci polymeru p i zv˝öené teplot . Reakcí HA s r zn˝mi aminy s vyuûitím 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorfolinium chloridu (DMTMM) byly p ipraveny t i finální látky, z nichû vöechny podléhaly degradaci znateln rychleji n û nativní HA. Charles University Faculty of Pharmacy in Hradec Králové Department of Organic and Bioorganic Chemistry Author: Gabriela Volfová Supervisors: Mgr. Radovan Bu a, Ph.D. doc. PharmDr. Jaroslav Roh, Ph.D. Title: Synthesis and study of hyaluronic acid derivatives modified on carboxyl Abstract: Hyaluronic acid (HA) is a naturally occurring linear polysaccharide. Due to its biocompatibility and non-immunogenicity, HA and its derivatives are widely used in various biological and medical applications. The e cacy of each formulation is strongly related to the molecular weight (MW) of the HA used. Unfortunately, various factors can a ect the MW during the manufacturing process. Elevated tem- perature required during the sterilization process is one significant example, which can lead to a reduction in molecular weight and result in a loss of some desired properties of the final product. This work focuses on the synthesis of HA amides formed on its free carboxyl group and subsequently studying the e ect of this modifi- cation on the degradation rate at elevated temperature. Three final compounds were prepared by reacting HA with various amines using 4-(4,6-dimethoxy-1,3,5-triazin-2- yl)-4-methylmorpholinium chloride (DMTMM), all of which underwent degradation noticeably faster than native HA.

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/179228

Permalink: http://www.nusl.cz/ntk/nusl-520824


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Rigorous theses