Název: Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering
Autoři: Beran, M. ; Berková, E. ; Musílková, Jana ; Sedlář, Antonín ; Slepička, P. ; Fajstavr, D.
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Conference on Nanomaterials - Research & Application /13./ NANOCON, Brno (CZ), 20211020
Rok: 2021
Jazyk: eng
Abstrakt: The purpose of the study was development of soft foams from resorbable polymers with unique micro-macro porous interconnected hierarchical structure specially designed as scaffold for engineering of soft tissues. The foams have been prepared by freeze-drying of solutions of polylactide (PLA) and polycaprolactone (PCL) in 1,4-dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose or NaCl porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the porogen crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were seeded with adipose-derived stem cells (ASC) and sarcoma osteogenic-2 (SaOs-2) human osteoblast-like cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test. Biocompatibility and usability of the scaffolds was successfully verified by incubation with adipose-derived stem cells and SaOs-2 human osteosarcoma cell line. Mineralised scaffolds are more suitable growth supports for both the cell types than unmineralized collagen scaffolds. The scaffolds have been specially designed for engineering of soft tissues, but they can be used in other categories of tissue engineering, too.
Klíčová slova: hierarchical structure; polycarolactone; polylactide; soft foams; tissue engineering
Číslo projektu: NU20-08-00208
Poskytovatel projektu: GA MZd
Zdrojový dokument: NANOCON 2021 - Conference proceedings, ISBN 978-80-88365-00-6, ISSN 2694-930X

Instituce: Fyziologický ústav AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: https://www.confer.cz/nanocon/2021/read/4346-mineralised-polylactide-and-polycaprolactone-soft-foams-with-hierarchical-micro-macro-porous-structure-for-tissue-engineering.pdf
Původní záznam: https://hdl.handle.net/11104/0338716

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-519931


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Fyziologický ústav
Konferenční materiály > Příspěvky z konference